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Title: The membrane effects of melittin on gastric and colorectal cancer.
Austin Authors: Soliman, Caroline;Eastwood, Sarah;Truong, Vi Khanh;Ramsland, Paul A ;Elbourne, Aaron
Affiliation: Department of Surgery, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia
School of Science, RMIT University, Bundoora West Campusm Bundoora, Victoria, Australia
Nanobiotechnology Laboratory, RMIT University, Melbourne City Campus, Melbourne, Victoria, Australia
Department of Immunology, Central Clinical School (Monash University), Alfred Medical Research and Education Precinct, Melbourne, Victoria, Australia
Issue Date: 17-Oct-2019
Date: 2019-10-17
Publication information: PLoS One 2019; 14(10): e0224028
Abstract: The cytotoxic effects of melittin, a bee-venom peptide, have been widely studied towards cancer cells. Typically, these studies have examined the effect of melittin over extended-time courses (6-24 hours), meaning that immediate cellular interactions have been overlooked. In this work, we demonstrate the rapid effects of melittin on both gastric and colorectal cancer, specifically AGS, COLO205 and HCT-15 cell lines, over a period of 15 minutes. Melittin exhibited a dose dependent effect at 4 hours of treatment, with complete cellular death occurring at the highest dose of 20 μg/mL. Interestingly, when observed at shorter time points, melittin induced cellular changes within seconds; membrane damage was observed as swelling, breakage or blebbing. High-resolution imaging revealed treated cells to be compromised, showing clear change in cellular morphology. After 1 minute of melittin treatment, membrane changes were observed, and intracellular material could be seen expelled from the cells. Overall, these results enhance our understanding of the fast acting anti-cancer effects of melittin.
DOI: 10.1371/journal.pone.0224028
ORCID: 0000-0002-2107-2738
Journal: PLoS One
PubMed URL: 31622415
Type: Journal Article
Appears in Collections:Journal articles

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