Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/21918
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dc.contributor.authorSoliman, Caroline-
dc.contributor.authorEastwood, Sarah-
dc.contributor.authorTruong, Vi Khanh-
dc.contributor.authorRamsland, Paul A-
dc.contributor.authorElbourne, Aaron-
dc.date2019-10-17-
dc.date.accessioned2019-10-20T22:40:32Z-
dc.date.available2019-10-20T22:40:32Z-
dc.date.issued2019-10-17-
dc.identifier.citationPLoS One 2019; 14(10): e0224028-
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/21918-
dc.description.abstractThe cytotoxic effects of melittin, a bee-venom peptide, have been widely studied towards cancer cells. Typically, these studies have examined the effect of melittin over extended-time courses (6-24 hours), meaning that immediate cellular interactions have been overlooked. In this work, we demonstrate the rapid effects of melittin on both gastric and colorectal cancer, specifically AGS, COLO205 and HCT-15 cell lines, over a period of 15 minutes. Melittin exhibited a dose dependent effect at 4 hours of treatment, with complete cellular death occurring at the highest dose of 20 μg/mL. Interestingly, when observed at shorter time points, melittin induced cellular changes within seconds; membrane damage was observed as swelling, breakage or blebbing. High-resolution imaging revealed treated cells to be compromised, showing clear change in cellular morphology. After 1 minute of melittin treatment, membrane changes were observed, and intracellular material could be seen expelled from the cells. Overall, these results enhance our understanding of the fast acting anti-cancer effects of melittin.-
dc.language.isoeng-
dc.titleThe membrane effects of melittin on gastric and colorectal cancer.-
dc.typeJournal Article-
dc.identifier.journaltitlePLoS One-
dc.identifier.affiliationDepartment of Surgery, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australiaen
dc.identifier.affiliationSchool of Science, RMIT University, Bundoora West Campusm Bundoora, Victoria, Australiaen
dc.identifier.affiliationNanobiotechnology Laboratory, RMIT University, Melbourne City Campus, Melbourne, Victoria, Australiaen
dc.identifier.affiliationDepartment of Immunology, Central Clinical School (Monash University), Alfred Medical Research and Education Precinct, Melbourne, Victoria, Australiaen
dc.identifier.doi10.1371/journal.pone.0224028-
dc.identifier.orcid0000-0002-2107-2738-
dc.identifier.orcid0000-0002-4472-4372-
dc.identifier.pubmedid31622415-
dc.type.austinJournal Article-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
crisitem.author.deptSurgery (University of Melbourne)-
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