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Title: Inhibition by a stable factor derived from neutrophils of endothelium-dependent relaxation in rat aorta.
Austin Authors: Liu, J J;Chen, J R;Wiley, J;Johnston, C C;Buxton, Brian F 
Affiliation: Department of Cardiac Surgery, University of Melbourne Austin Hospital, Heidelberg, Victoria, Australia
Issue Date: 1-Oct-1993
Publication information: The American Journal of Physiology; 265(4 Pt 2): H1454-9
Abstract: Polymorphonuclear leukocytes (PMNs) may play an important role in many pathophysiological states. The effect of a factor derived from PMNs on endothelium-dependent relaxation was studied using rat aortic rings in organ chambers. PMNs were obtained from cardiac surgical patients and healthy volunteers. After incubation in Krebs solution for 3 h, supernatants of PMN suspensions were isolated and used to pretreat the aortic rings for 30 min. The results showed that the supernatants derived from 1 x 10(4) to 5 x 10(6) cells/ml PMNs produced a concentration-dependent inhibition of endothelium-dependent relaxation to acetylcholine but not endothelium-independent relaxation to sodium nitroprusside. The effect could not be prevented by oxygen free radical scavenger superoxide dismutase (150 U/ml), catalase (1,200 U/ml), or mannitol (20 mM) used alone or in combination. Heating the supernatants at 95 degrees C for 30 min did not reduce the inhibitory effect. L-Arginine at 3 x 10(-5) to 3 x 10(-3) M did not significantly reverse the inhibitory effect of the PMN-derived factor. In conclusion, this study reveals that a heat-stable factor derived from human PMNs potently inhibits acetylcholine-induced endothelium-dependent relaxation but not sodium nitroprusside-induced endothelium-independent relaxation in rat aorta. This inhibitory effect is not caused by oxygen free radicals, a limitation of nitric oxide precursor or other unstable factors.
Gov't Doc #: 8238434
Journal: American Journal of Physiology
Type: Journal Article
Subjects: Animals
Aorta.drug effects
Drug Stability
Endothelium, Vascular.drug effects
Hot Temperature
In Vitro Techniques
Vasodilation.drug effects
Appears in Collections:Journal articles

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