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dc.contributor.authorLiu, J Jen
dc.contributor.authorChen, J Ren
dc.contributor.authorWiley, Jen
dc.contributor.authorJohnston, C Cen
dc.contributor.authorBuxton, Brian Fen
dc.identifier.citationThe American Journal of Physiology; 265(4 Pt 2): H1454-9en
dc.description.abstractPolymorphonuclear leukocytes (PMNs) may play an important role in many pathophysiological states. The effect of a factor derived from PMNs on endothelium-dependent relaxation was studied using rat aortic rings in organ chambers. PMNs were obtained from cardiac surgical patients and healthy volunteers. After incubation in Krebs solution for 3 h, supernatants of PMN suspensions were isolated and used to pretreat the aortic rings for 30 min. The results showed that the supernatants derived from 1 x 10(4) to 5 x 10(6) cells/ml PMNs produced a concentration-dependent inhibition of endothelium-dependent relaxation to acetylcholine but not endothelium-independent relaxation to sodium nitroprusside. The effect could not be prevented by oxygen free radical scavenger superoxide dismutase (150 U/ml), catalase (1,200 U/ml), or mannitol (20 mM) used alone or in combination. Heating the supernatants at 95 degrees C for 30 min did not reduce the inhibitory effect. L-Arginine at 3 x 10(-5) to 3 x 10(-3) M did not significantly reverse the inhibitory effect of the PMN-derived factor. In conclusion, this study reveals that a heat-stable factor derived from human PMNs potently inhibits acetylcholine-induced endothelium-dependent relaxation but not sodium nitroprusside-induced endothelium-independent relaxation in rat aorta. This inhibitory effect is not caused by oxygen free radicals, a limitation of nitric oxide precursor or other unstable factors.en
dc.subject.otherAorta.drug effectsen
dc.subject.otherDrug Stabilityen
dc.subject.otherEndothelium, Vascular.drug effectsen
dc.subject.otherHot Temperatureen
dc.subject.otherIn Vitro Techniquesen
dc.subject.otherVasodilation.drug effectsen
dc.titleInhibition by a stable factor derived from neutrophils of endothelium-dependent relaxation in rat aorta.en
dc.typeJournal Articleen
dc.identifier.journaltitleAmerican Journal of Physiologyen
dc.identifier.affiliationDepartment of Cardiac Surgery, University of Melbourne Austin Hospital, Heidelberg, Victoria, Australiaen
dc.type.austinJournal Articleen
item.fulltextNo Fulltext-
item.openairetypeJournal Article- Surgery-
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