Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13285
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dc.contributor.authorLiu, J Jen
dc.contributor.authorChen, J Ren
dc.contributor.authorWiley, Jen
dc.contributor.authorJohnston, C Cen
dc.contributor.authorBuxton, Brian Fen
dc.date.accessioned2015-05-16T03:06:34Z
dc.date.available2015-05-16T03:06:34Z
dc.date.issued1993-10-01en
dc.identifier.citationThe American Journal of Physiology; 265(4 Pt 2): H1454-9en
dc.identifier.govdoc8238434en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/13285en
dc.description.abstractPolymorphonuclear leukocytes (PMNs) may play an important role in many pathophysiological states. The effect of a factor derived from PMNs on endothelium-dependent relaxation was studied using rat aortic rings in organ chambers. PMNs were obtained from cardiac surgical patients and healthy volunteers. After incubation in Krebs solution for 3 h, supernatants of PMN suspensions were isolated and used to pretreat the aortic rings for 30 min. The results showed that the supernatants derived from 1 x 10(4) to 5 x 10(6) cells/ml PMNs produced a concentration-dependent inhibition of endothelium-dependent relaxation to acetylcholine but not endothelium-independent relaxation to sodium nitroprusside. The effect could not be prevented by oxygen free radical scavenger superoxide dismutase (150 U/ml), catalase (1,200 U/ml), or mannitol (20 mM) used alone or in combination. Heating the supernatants at 95 degrees C for 30 min did not reduce the inhibitory effect. L-Arginine at 3 x 10(-5) to 3 x 10(-3) M did not significantly reverse the inhibitory effect of the PMN-derived factor. In conclusion, this study reveals that a heat-stable factor derived from human PMNs potently inhibits acetylcholine-induced endothelium-dependent relaxation but not sodium nitroprusside-induced endothelium-independent relaxation in rat aorta. This inhibitory effect is not caused by oxygen free radicals, a limitation of nitric oxide precursor or other unstable factors.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherAorta.drug effectsen
dc.subject.otherArginine.pharmacologyen
dc.subject.otherDrug Stabilityen
dc.subject.otherEndothelium, Vascular.drug effectsen
dc.subject.otherHot Temperatureen
dc.subject.otherHumansen
dc.subject.otherIn Vitro Techniquesen
dc.subject.otherNeutrophils.metabolismen
dc.subject.otherRatsen
dc.subject.otherVasodilation.drug effectsen
dc.titleInhibition by a stable factor derived from neutrophils of endothelium-dependent relaxation in rat aorta.en
dc.typeJournal Articleen
dc.identifier.journaltitleAmerican Journal of Physiologyen
dc.identifier.affiliationDepartment of Cardiac Surgery, University of Melbourne Austin Hospital, Heidelberg, Victoria, Australiaen
dc.description.pagesH1454-9en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/8238434en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptCardiac Surgery-
Appears in Collections:Journal articles
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