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Title: Co-localization of angiotensin-converting enzyme 2-, octomer-4- and CD34-positive cells in rabbit atherosclerotic plaques.
Austin Authors: Zulli, Anthony;Rai, Sudarshan;Buxton, Brian F ;Burrell, Louise M ;Hare, David L 
Affiliation: Department of Medicine, University of Melbourne, Austin Health, Heidelberg, Melbourne, Victoria, Australia
Issue Date: 11-Jan-2008
Publication information: Experimental Physiology 2008; 93(5): 564-9
Abstract: Angiotensin-converting enzyme 2 (ACE2) is a novel enzyme with possible implications in the treatment of blood pressure disorders. Recent evidence suggests that an upregulation of ACE2 can be stimulated by all-trans retinoic acid (at-RA); however, at-RA also affects regulation of the stem-cell marker octomer-4 (Oct-4) and thus cellular differentiation. We have previously shown that smooth muscle cells and macrophages present within rabbit atherosclerotic plaques are positive for ACE2, Oct-4 and the haematopoietic stem-cell marker CD34. Thus, to provide evidence that possible at-RA treatment could affect both plaque cellular biology (via effects on cellular differentiation) and blood pressure (via ACE2), it is vital to show that cells with atherosclerotic plaques co-express all three markers. Thus, we sought to provide evidence that a subset of cells within atherosclerotic plaques is positive for ACE2, Oct-4 and CD34. We used New Zealand White rabbits that were fed a control diet supplemented with 0.5% cholesterol plus 1% methionine for 4 weeks and then allowed to consume a normal diet for 10 weeks. Immunohistochemistry was performed by standard techniques. We report that ACE2, Oct-4 and CD34 were all present within atherosclerotic plaques. Although macrophages were positive for all three markers, spindle-shaped cells in the media did not show all three markers. The endothelium overlying normal arterial wall showed positive Oct-4 and ACE2 immunoreactivity, but CD34 immunoreactivity was patchy, indicating that such cells might not have fully differentiated. It is concluded that cells in atherosclerotic plaques express co-express ACE2, Oct-4 and CD34. Further studies aimed at establishing the effects of all-trans retinoic acid on blood pressure and atherosclerotic cell differentiation are warranted.
Gov't Doc #: 18192339
DOI: 10.1113/expphysiol.2007.040204
ORCID: 0000-0001-9554-6556
PubMed URL: 18192339
Type: Journal Article
Subjects: Animals
Antigens, CD34.metabolism
Aorta, Abdominal.pathology
Endothelium, Vascular.metabolism.pathology
Octamer Transcription Factor-3.metabolism
Peptidyl-Dipeptidase A.metabolism
Appears in Collections:Journal articles

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