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Title: Determination of nefazodone and its pharmacologically active metabolites in human blood plasma and breast milk by high-performance liquid chromatography.
Austin Authors: Dodd, Seetal;Buist, Anne ;Burrows, Graham D;Maguire, K P;Norman, Trevor R 
Affiliation: Department of Psychiatry, University of Melbourne, Austin and Repatriation Medical Centre, Victoria, Australia
Issue Date: 9-Jul-1999
Publication information: Journal of Chromatography. B, Biomedical Sciences and Applications; 730(2): 249-55
Abstract: A method is described for the determination of nefazodone and its active metabolites hydroxynefazodone, the dione BMS-180492 and m-chlorophenylpiperazine in blood plasma and expressed human milk based on reversed-phase high-performance liquid chromatography. Measurements were performed on drug-free plasma and expressed human milk spiked with nefazodone and metabolites to prepare and validate standard curves and specimens collected from nursing mothers. Parent drug and metabolites were separated from the biological matrices by solid-phase extraction using CERTIFY columns. Chromatographic separation was achieved with a C18 column and compounds were detected by their absorbance at 205 nm. Trazodone was used as an internal standard. The assay was validated for each analyte in the concentration range 200 to 1200 ng/ml.
Gov't Doc #: 10448960
Journal: Journal of chromatography. B, Biomedical sciences and applications
Type: Journal Article
Subjects: Antidepressive Agents, Second-Generation.analysis.blood
Chromatography, High Pressure Liquid.methods
Milk, Human.chemistry
Reference Standards
Reproducibility of Results
Appears in Collections:Journal articles

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