Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/32834
Title: Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review.
Austin Authors: Rangamuwa, Kanishka;Aloe, Christian;Christie, Michael;Asselin-Labat, Marie-Liesse;Batey, Daniel;Irving, Lou;John, Tom ;Bozinovski, Steven;Leong, Tracy L ;Steinfort, Daniel
Affiliation: Department of Respiratory Medicine, Royal Melbourne Hospital, Melbourne, VIC, Australia
School of Health and Biomedical Sciences, RMIT University, Bundoora, VIC, Australia.
Department of Pathology, Royal Melbourne Hospital, Melbourne, VIC, Australia.
Personalised Oncology Division, Walter Eliza Hall Institute, Melbourne, VIC, Australia.
Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.
Department of Medicine Royal Melbourne Hospital (RMH), University of Melbourne, Parkville, VIC, Australia.
Respiratory and Sleep Medicine
Issue Date: Apr-2023
Date: 2023
Publication information: Frontiers in Oncology 2023; 13
Abstract: Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer death worldwide. Immunotherapy with immune checkpoint inhibitors (ICI) has significantly improved outcomes in some patients, however 80-85% of patients receiving immunotherapy develop primary resistance, manifesting as a lack of response to therapy. Of those that do have an initial response, disease progression may occur due to acquired resistance. The make-up of the tumour microenvironment (TME) and the interaction between tumour infiltrating immune cells and cancer cells can have a large impact on the response to immunotherapy. Robust assessment of the TME with accurate and reproducible methods is vital to understanding mechanisms of immunotherapy resistance. In this paper we will review the evidence of several methodologies to assess the TME, including multiplex immunohistochemistry, imaging mass cytometry, flow cytometry, mass cytometry and RNA sequencing.
URI: https://ahro.austin.org.au/austinjspui/handle/1/32834
DOI: 10.3389/fonc.2023.1129195
ORCID: 
Journal: Frontiers in Oncology
Start page: 1129195
PubMed URL: 37143952
Type: Journal Article
Subjects: RNAseq analysis
cytometry
immune interaction
immunohistochemistry
immunotherapy
non-smal cell lung cancer
tumour microenvironment
Appears in Collections:Journal articles

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