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https://ahro.austin.org.au/austinjspui/handle/1/31966| Title: | Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry. | Austin Authors: | Quigley, Luke T;Pang, Lokman;Tavancheh, Elnaz;Ernst, Matthias ;Behren, Andreas;Huynh, Jennifer;Da Gama Duarte, Jessica | Affiliation: | Department of Medicine, University of Melbourne, Parkville, VIC 3010, Australia. Olivia Newton-John Cancer Research Institute |
Issue Date: | 17-Mar-2023 | Date: | 2023 | Publication information: | STAR Protocols 2023-03-17; 4(1) | Abstract: | Tertiary lymphoid structures (TLSs) are ectopic lymphoid aggregates that can develop within or adjacent to tumors, but protocols that can accurately identify and characterize TLSs are lacking. Here, we present a protocol for the in situ interrogation and characterization of TLSs in human and murine tissue sections using Opal™-tyramide signal amplification multiplex immunohistochemistry. This protocol enables simultaneous detection of up to 7 markers (6 antigens and a DAPI counterstain). We also describe a grading system to identify immature and mature TLSs. | URI: | https://ahro.austin.org.au/austinjspui/handle/1/31966 | DOI: | 10.1016/j.xpro.2022.101961 | ORCID: | Journal: | STAR Protocols | Start page: | 101961 | PubMed URL: | 36633948 | PubMed URL: | https://pubmed.ncbi.nlm.nih.gov/36633948/ | ISSN: | 2666-1667 | Type: | Journal Article | Subjects: | Cancer Immunology Microscopy |
| Appears in Collections: | Journal articles |
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