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|Title:||Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry.||Austin Authors:||Quigley, Luke T;Pang, Lokman;Tavancheh, Elnaz;Ernst, Matthias ;Behren, Andreas;Huynh, Jennifer;Da Gama Duarte, Jessica||Affiliation:||Department of Medicine, University of Melbourne, Parkville, VIC 3010, Australia.
Olivia Newton-John Cancer Research Institute
|Issue Date:||17-Mar-2023||Date:||2023||Publication information:||STAR Protocols 2023-03-17; 4(1)||Abstract:||Tertiary lymphoid structures (TLSs) are ectopic lymphoid aggregates that can develop within or adjacent to tumors, but protocols that can accurately identify and characterize TLSs are lacking. Here, we present a protocol for the in situ interrogation and characterization of TLSs in human and murine tissue sections using Opal™-tyramide signal amplification multiplex immunohistochemistry. This protocol enables simultaneous detection of up to 7 markers (6 antigens and a DAPI counterstain). We also describe a grading system to identify immature and mature TLSs.||URI:||https://ahro.austin.org.au/austinjspui/handle/1/31966||DOI:||10.1016/j.xpro.2022.101961||ORCID:||Journal:||STAR Protocols||Start page:||101961||PubMed URL:||36633948||PubMed URL:||https://pubmed.ncbi.nlm.nih.gov/36633948/||ISSN:||2666-1667||Type:||Journal Article||Subjects:||Cancer
|Appears in Collections:||Journal articles|
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checked on Dec 7, 2023
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