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Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/31966
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dc.contributor.authorQuigley, Luke T-
dc.contributor.authorPang, Lokman-
dc.contributor.authorTavancheh, Elnaz-
dc.contributor.authorErnst, Matthias-
dc.contributor.authorBehren, Andreas-
dc.contributor.authorHuynh, Jennifer-
dc.contributor.authorDa Gama Duarte, Jessica-
dc.date2023-
dc.date.accessioned2023-01-24T03:01:18Z-
dc.date.available2023-01-24T03:01:18Z-
dc.date.issued2023-03-17-
dc.identifier.citationSTAR Protocols 2023-03-17; 4(1)en_US
dc.identifier.issn2666-1667-
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/31966-
dc.description.abstractTertiary lymphoid structures (TLSs) are ectopic lymphoid aggregates that can develop within or adjacent to tumors, but protocols that can accurately identify and characterize TLSs are lacking. Here, we present a protocol for the in situ interrogation and characterization of TLSs in human and murine tissue sections using Opal™-tyramide signal amplification multiplex immunohistochemistry. This protocol enables simultaneous detection of up to 7 markers (6 antigens and a DAPI counterstain). We also describe a grading system to identify immature and mature TLSs.en_US
dc.language.isoeng-
dc.subjectCanceren_US
dc.subjectImmunologyen_US
dc.subjectMicroscopyen_US
dc.titleProtocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry.en_US
dc.typeJournal Articleen_US
dc.identifier.journaltitleSTAR Protocolsen_US
dc.identifier.affiliationDepartment of Medicine, University of Melbourne, Parkville, VIC 3010, Australia.en_US
dc.identifier.affiliationOlivia Newton-John Cancer Research Instituteen_US
dc.identifier.pubmedurihttps://pubmed.ncbi.nlm.nih.gov/36633948/en_US
dc.identifier.doi10.1016/j.xpro.2022.101961en_US
dc.type.contentTexten_US
dc.identifier.pubmedid36633948-
dc.description.volume4-
dc.description.issue1-
dc.description.startpage101961-
local.name.researcherErnst, Matthias-
item.languageiso639-1en-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.grantfulltextnone-
crisitem.author.deptOlivia Newton-John Cancer Research Institute-
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