Please use this identifier to cite or link to this item:
https://ahro.austin.org.au/austinjspui/handle/1/30484| Title: | Establishing a multiplex imaging panel to study T cell development in the thymus in mouse. | Austin Authors: | Allam, Amr H;Russell, Sarah M | Affiliation: | Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, VIC 3000, Australia.. Olivia Newton-John Cancer Research Institute Immune Signalling Laboratory, Peter MacCallum Cancer Centre, Melbourne, VIC 3000 Australia.. Optical Sciences Centre, School of Science Computing and Engineering Technologies, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.. La Trobe University School of Cancer Medicine, Heidelberg, VIC, 3084, Australia.. |
Issue Date: | 21-Jun-2022 | Date: | 2022-09-16 | Publication information: | STAR protocols 2022; 3(3): 101472 | Abstract: | Multiplexed immunohistochemistry enables analysis of cellular and signaling events in the context of an intact organ. Here, we describe protocols for applying multiplexed immunohistochemistry to the mouse thymus. In particular, we describe how to identify cells at the specific differentiation stage known as β-selection, and to monitor pre-TCR signaling and the cellular response at that stage. For complete details on the use and execution of this protocol, please refer to Allam et al. (2021). | URI: | https://ahro.austin.org.au/austinjspui/handle/1/30484 | DOI: | 10.1016/j.xpro.2022.101472 | ORCID: | 0000-0002-8743-3639 0000-0001-5826-9641 |
Journal: | STAR protocols | PubMed URL: | 35769925 | PubMed URL: | https://pubmed.ncbi.nlm.nih.gov/35769925/ | Type: | Journal Article | Subjects: | Cell Biology Immunology Microscopy |
| Appears in Collections: | Journal articles |
Show full item record
Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.