Establishing a multiplex imaging panel to study T cell development in the thymus in mouse.

2022-06-21

Allam, Amr H

Russell, Sarah M

Cell Biology

Immunology

Microscopy

Journal Article

0000-0002-8743-3639

0000-0001-5826-9641

10.1016/j.xpro.2022.101472

Multiplexed immunohistochemistry enables analysis of cellular and signaling events in the context of an intact organ. Here, we describe protocols for applying multiplexed immunohistochemistry to the mouse thymus. In particular, we describe how to identify cells at the specific differentiation stage known as β-selection, and to monitor pre-TCR signaling and the cellular response at that stage. For complete details on the use and execution of this protocol, please refer to Allam et al. (2021).

link

STAR protocols 2022; 3(3): 101472

STAR protocols