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Title: Establishing a multiplex imaging panel to study T cell development in the thymus in mouse.
Austin Authors: Allam, Amr H;Russell, Sarah M
Affiliation: Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, VIC 3000, Australia..
Olivia Newton-John Cancer Research Institute
Immune Signalling Laboratory, Peter MacCallum Cancer Centre, Melbourne, VIC 3000 Australia..
Optical Sciences Centre, School of Science Computing and Engineering Technologies, Swinburne University of Technology, Hawthorn, VIC 3122, Australia..
La Trobe University School of Cancer Medicine, Heidelberg, VIC, 3084, Australia..
Issue Date: 21-Jun-2022
Date: 2022-09-16
Publication information: STAR protocols 2022; 3(3): 101472
Abstract: Multiplexed immunohistochemistry enables analysis of cellular and signaling events in the context of an intact organ. Here, we describe protocols for applying multiplexed immunohistochemistry to the mouse thymus. In particular, we describe how to identify cells at the specific differentiation stage known as β-selection, and to monitor pre-TCR signaling and the cellular response at that stage. For complete details on the use and execution of this protocol, please refer to Allam et al. (2021).
DOI: 10.1016/j.xpro.2022.101472
ORCID: 0000-0002-8743-3639
Journal: STAR protocols
PubMed URL: 35769925
PubMed URL:
Type: Journal Article
Subjects: Cell Biology
Appears in Collections:Journal articles

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