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Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/30484
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dc.contributor.authorAllam, Amr H-
dc.contributor.authorRussell, Sarah M-
dc.date2022-09-16-
dc.date.accessioned2022-07-06T06:23:31Z-
dc.date.available2022-07-06T06:23:31Z-
dc.date.issued2022-06-21-
dc.identifier.citationSTAR protocols 2022; 3(3): 101472en
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/30484-
dc.description.abstractMultiplexed immunohistochemistry enables analysis of cellular and signaling events in the context of an intact organ. Here, we describe protocols for applying multiplexed immunohistochemistry to the mouse thymus. In particular, we describe how to identify cells at the specific differentiation stage known as β-selection, and to monitor pre-TCR signaling and the cellular response at that stage. For complete details on the use and execution of this protocol, please refer to Allam et al. (2021).en
dc.language.isoeng
dc.subjectCell Biologyen
dc.subjectImmunologyen
dc.subjectMicroscopyen
dc.titleEstablishing a multiplex imaging panel to study T cell development in the thymus in mouse.en
dc.typeJournal Articleen
dc.identifier.journaltitleSTAR protocolsen
dc.identifier.affiliationSir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, VIC 3000, Australia..en
dc.identifier.affiliationOlivia Newton-John Cancer Research Instituteen
dc.identifier.affiliationImmune Signalling Laboratory, Peter MacCallum Cancer Centre, Melbourne, VIC 3000 Australia..en
dc.identifier.affiliationOptical Sciences Centre, School of Science Computing and Engineering Technologies, Swinburne University of Technology, Hawthorn, VIC 3122, Australia..en
dc.identifier.affiliationLa Trobe University School of Cancer Medicine, Heidelberg, VIC, 3084, Australia..en
dc.identifier.pubmedurihttps://pubmed.ncbi.nlm.nih.gov/35769925/en
dc.identifier.doi10.1016/j.xpro.2022.101472en
dc.type.contentTexten
dc.identifier.orcid0000-0002-8743-3639en
dc.identifier.orcid0000-0001-5826-9641en
dc.identifier.pubmedid35769925
item.fulltextNo Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
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