Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/30263
Title: SARS-CoV-2 infection results in immune responses in the respiratory tract and peripheral blood that suggest mechanisms of disease severity.
Austin Authors: Zhang, Wuji;Chua, Brendon Y;Selva, Kevin J;Kedzierski, Lukasz;Ashhurst, Thomas M;Haycroft, Ebene R;Shoffner-Beck, Suzanne K;Hensen, Luca;Boyd, David F;James, Fiona L ;Mouhtouris, Effie ;Kwong, Jason C ;Chua, Kyra Y L ;Drewett, George P ;Copaescu, Ana ;Dobson, Julie E ;Rowntree, Louise C;Habel, Jennifer R;Allen, Lilith F;Koay, Hui-Fern;Neil, Jessica A;Gartner, Matthew J;Lee, Christina Y;Andersson, Patiyan;Khan, Sadid F;Blakeway, Luke;Wisniewski, Jessica;McMahon, James H;Vine, Erica E;Cunningham, Anthony L;Audsley, Jennifer;Thevarajan, Irani;Seemann, Torsten;Sherry, Norelle L ;Amanat, Fatima;Krammer, Florian;Londrigan, Sarah L;Wakim, Linda M;King, Nicholas J C;Godfrey, Dale I;Mackay, Laura K;Thomas, Paul G;Nicholson, Suellen;Arnold, Kelly B;Chung, Amy W;Holmes, Natasha E ;Smibert, Olivia C ;Trubiano, Jason ;Gordon, Claire L ;Nguyen, Thi H O;Kedzierska, Katherine
Affiliation: Victorian Infectious Diseases Services, The Royal Melbourne Hospital and Doherty Department University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, 3000, VIC, Australia..
Department of Infectious Diseases, Monash University and Alfred Hospital, Melbourne, VIC, Australia..
Department of Infectious Diseases, Monash Medical Centre, Melbourne, VIC, Australia..
Centre for Virus Research, The Westmead Institute for Medical Research, Westmead, NSW, Australia..
School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, NSW, Australia..
Sydney Infectious Diseases, Faculty of Medicine and Health, University of Sydney, Westmead, NSW, Australia..
Infectious Diseases
Department of Infectious Diseases, Peter McCallum Cancer Centre, Melbourne, VIC, Australia..
National Centre for Infections in Cancer, Peter McCallum Cancer Centre, Melbourne, VIC, Australia..
Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia..
Sydney Cytometry Core Research Facility, Charles Perkins Centre, Centenary Institute and University of Sydney, Sydney, NSW, Australia..
Sydney Institute for Infectious Diseases, University of Sydney, Sydney, NSW, Australia..
Radiology
Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, Victoria, 3000, Australia..
Department of Critical Care, University of Melbourne, Parkville, VIC, Australia..
Data Analytics Research and Evaluation (DARE) Centre
Centre for Antibiotic Allergy and Research
Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan..
Department of Infectious Diseases, Peter McCallum Cancer Centre, Melbourne, VIC, Australia..
Medicine (University of Melbourne)
Charles Perkins Centre, University of Sydney, Sydney, NSW, Australia..
Viral Immunopathology Laboratory, Discipline of Pathology, School of Medical Sciences, University of Sydney, Sydney, NSW, Australia..
Sydney Nano, University of Sydney, Sydney, NSW, 2006, Australia..
Victorian Infectious Diseases Reference Laboratory, The Royal Melbourne Hospital at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia..
Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology & Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia..
Department of Infectious Diseases, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, 3000, Australia..
Department of Biomedical Engineering, University of Michigan, Michigan, USA..
Department of Immunology, St Jude Children's Research Hospital, Memphis, TN, USA..
Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA..
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA..
Department of Biomedical Engineering, University of Michigan, Michigan, USA..
Issue Date: 19-May-2022
Date: 2022
Publication information: Nature communications 2022; 13(1): 2774
Abstract: Respiratory tract infection with SARS-CoV-2 results in varying immunopathology underlying COVID-19. We examine cellular, humoral and cytokine responses covering 382 immune components in longitudinal blood and respiratory samples from hospitalized COVID-19 patients. SARS-CoV-2-specific IgM, IgG, IgA are detected in respiratory tract and blood, however, receptor-binding domain (RBD)-specific IgM and IgG seroconversion is enhanced in respiratory specimens. SARS-CoV-2 neutralization activity in respiratory samples correlates with RBD-specific IgM and IgG levels. Cytokines/chemokines vary between respiratory samples and plasma, indicating that inflammation should be assessed in respiratory specimens to understand immunopathology. IFN-α2 and IL-12p70 in endotracheal aspirate and neutralization in sputum negatively correlate with duration of hospital stay. Diverse immune subsets are detected in respiratory samples, dominated by neutrophils. Importantly, dexamethasone treatment does not affect humoral responses in blood of COVID-19 patients. Our study unveils differential immune responses between respiratory samples and blood, and shows how drug therapy affects immune responses during COVID-19.
URI: https://ahro.austin.org.au/austinjspui/handle/1/30263
DOI: 10.1038/s41467-022-30088-y
ORCID: http://orcid.org/0000-0001-7460-4956
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Journal: Nature communications
PubMed URL: 35589689
PubMed URL: https://pubmed.ncbi.nlm.nih.gov/35589689/
Type: Journal Article
Appears in Collections:Journal articles

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