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Title: Profiling the glycome of Cardicola forsteri, a blood fluke parasitic to bluefin tuna.
Austin Authors: Coff, Lachlan;Abrahams, Jodie L;Collett, Simon;Power, Cecilia;Nowak, Barbara F;Kolarich, Daniel;Bott, Nathan J;Ramsland, Paul A 
Affiliation: School of Science, STEM College, RMIT University, Bundoora, VIC 3083, Australia..
Institute for Glycomics, Griffith University, Southport, QLD 4215, Australia..
Institute for Marine and Antarctic Studies, University of Tasmania, Locked Bag 1370, Launceston, TAS 7250, Australia..
Department of Immunology, Monash University, Melbourne, VIC 3004, Australia;
Surgery (University of Melbourne)
ARC Centre of Excellence for Nanoscale BioPhotonics, Griffith University, Southport, QLD 4215, Australia..
Issue Date: Jan-2022
Date: 2021
Publication information: International journal for parasitology 2022; 52(1): 1-12
Abstract: Infections by blood flukes (Cardicola spp.) are considered the most significant health issue for ranched bluefin tuna, a major aquaculture industry in Japan and Australia. The host-parasite interfaces of trematodes, namely their teguments, are particularly rich in carbohydrates, which function both in evasion and modulation of the host immune system, while some are primary antigenic targets. In this study, histochemistry and mass spectrometry techniques were used to profile the glycans of Cardicola forsteri. Fluorescent lectin staining of adult flukes indicates the presence of oligomannose (Concanavalin A-reactive) and fucosylated (Pisum sativum agglutinin-reactive) N-glycans. Additionally, reactivity of succinylated wheat germ agglutinin (s-WGA) was localised to several internal organs of the digestive and monoecious reproductive systems. Glycan structures were further investigated with tandem mass spectrometry, which revealed structures indicated by lectin reactivity. While O-glycans from these adult specimens were not detectable by mass spectrometry, several oligomannose, paucimannosidic, and complex-type N-glycans were identified, including some carrying hexuronic acid and many carrying core xylose. This is, to our knowledge, the first glycomic characterisation of a marine platyhelminth, with broader implications for research into other trematodes.
DOI: 10.1016/j.ijpara.2021.06.004
ORCID: 0000-0002-2107-2738
Journal: International journal for parasitology
PubMed URL: 34391752
PubMed URL:
Type: Journal Article
Subjects: Aquaculture
Mass spectrometry
Appears in Collections:Journal articles

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