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Title: Zinc Preconditioning Provides Cytoprotection following Iodinated Contrast Media Exposure in In Vitro Models.
Austin Authors: Perera, Marlon ;Ischia, Joseph J ;Bolton, Damien M ;Shulkes, Arthur;Baldwin, Graham S;Patel, Oneel
Affiliation: Olivia Newton-John Cancer Research Institute..
Surgery (University of Melbourne)..
Issue Date: 17-Feb-2021
Date: 2021
Publication information: Contrast media & molecular imaging 2021; 2021: 6686803
Abstract: Normal human proximal renal kidney cells (HK-2) were preconditioned with either increasing doses of ZnCl2 or control. Following this preconditioning, cells were exposed to increasing concentrations of Iohexol 300 mg I2/ml for four hours. Key outcome measures included cell survival (MTT colorimetric assay) and ROS generation (H2DCFDA fluorescence assay). Contrast media induced a dose-dependent reduction in survival of HK-2 cells. Compared to control, contrast media at 150, 225, and 300 mg I2/ml resulted in 69.5% (SD 8.8%), 37.3% (SD 4.8%), and 4.8% (SD 6.6%) cell survival, respectively (p < 0.001). Preconditioning with 37.5 μM and 50 μM ZnCl2 increased cell survival by 173% (SD 27.8%) (p < 0.001) and 219% (SD 32.2%) (p < 0.001), respectively, compared to control preconditioning. Zinc preconditioning resulted in a reduction of ROS generation. Zinc pre-conditioning with 37.5 μM μM ZnCl2 reduced ROS generation by 46% (p < 0.001) compared to control pre-conditioning. Zinc preconditioning reduces oxidative stress following exposure to radiographic contrast media which in turn results in increased survival of renal cells. Translation of this in vitro finding in animal models will lay the foundation for future use of zinc preconditioning against contrast induced nephropathy.
DOI: 10.1155/2021/6686803
ORCID: 0000-0002-1138-6389
Journal: Contrast media & molecular imaging
PubMed URL: 33679268
PubMed URL:
Type: Journal Article
Appears in Collections:Journal articles

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