Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/28469
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dc.contributor.authorPerera, Marlon-
dc.contributor.authorIschia, Joseph J-
dc.contributor.authorBolton, Damien M-
dc.contributor.authorShulkes, Arthur-
dc.contributor.authorBaldwin, Graham S-
dc.contributor.authorPatel, Oneel-
dc.date2021-
dc.date.accessioned2022-01-10T03:24:46Z-
dc.date.available2022-01-10T03:24:46Z-
dc.date.issued2021-02-17-
dc.identifier.citationContrast media & molecular imaging 2021; 2021: 6686803en
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/28469-
dc.description.abstractNormal human proximal renal kidney cells (HK-2) were preconditioned with either increasing doses of ZnCl2 or control. Following this preconditioning, cells were exposed to increasing concentrations of Iohexol 300 mg I2/ml for four hours. Key outcome measures included cell survival (MTT colorimetric assay) and ROS generation (H2DCFDA fluorescence assay). Contrast media induced a dose-dependent reduction in survival of HK-2 cells. Compared to control, contrast media at 150, 225, and 300 mg I2/ml resulted in 69.5% (SD 8.8%), 37.3% (SD 4.8%), and 4.8% (SD 6.6%) cell survival, respectively (p < 0.001). Preconditioning with 37.5 μM and 50 μM ZnCl2 increased cell survival by 173% (SD 27.8%) (p < 0.001) and 219% (SD 32.2%) (p < 0.001), respectively, compared to control preconditioning. Zinc preconditioning resulted in a reduction of ROS generation. Zinc pre-conditioning with 37.5 μM μM ZnCl2 reduced ROS generation by 46% (p < 0.001) compared to control pre-conditioning. Zinc preconditioning reduces oxidative stress following exposure to radiographic contrast media which in turn results in increased survival of renal cells. Translation of this in vitro finding in animal models will lay the foundation for future use of zinc preconditioning against contrast induced nephropathy.en
dc.language.isoeng
dc.titleZinc Preconditioning Provides Cytoprotection following Iodinated Contrast Media Exposure in In Vitro Models.en
dc.typeJournal Articleen
dc.identifier.journaltitleContrast media & molecular imagingen
dc.identifier.affiliationOlivia Newton-John Cancer Research Institute..en
dc.identifier.affiliationSurgery (University of Melbourne)..en
dc.identifier.pubmedurihttps://pubmed.ncbi.nlm.nih.gov/33679268/en
dc.identifier.doi10.1155/2021/6686803en
dc.type.contentTexten
dc.identifier.orcid0000-0002-1138-6389en
dc.identifier.orcid0000-0001-7177-3631en
dc.identifier.orcid0000-0002-5145-6783en
dc.identifier.orcid0000-0002-0944-8747en
dc.identifier.orcid0000-0001-5628-7205en
dc.identifier.pubmedid33679268
local.name.researcherBolton, Damien M
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
crisitem.author.deptSurgery-
crisitem.author.deptUrology-
crisitem.author.deptUrology-
crisitem.author.deptUrology-
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