Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/26239
Title: Core Alzheimer's disease cerebrospinal fluid biomarker assays are not affected by aspiration or gravity drip extraction methods.
Austin Authors: Doecke, James D;Francois, Cindy;Fowler, Christopher J;Stoops, Erik;Bourgeat, Pierrick;Rainey-Smith, Stephanie R;Li, Qiao-Xin;Masters, Colin L ;Martins, Ralph N;Villemagne, Victor L ;Collins, Steven J;Vanderstichele, Hugo Marcel
Affiliation: Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA, USA
Edith Cowan University, Perth, WA, Australia
Australian Alzheimer's Research Foundation, Perth, WA, Australia
Centre for Healthy Ageing, Health Futures Institute, Murdoch University, Murdoch, WA, Australia
The Australian e-Health Research Centre, CSIRO, Brisbane, QLD, Australia
ADx NeuroSciences, Ghent, Belgium
University of Melbourne, Melbourne, VIC, Australia
Molecular Imaging and Therapy
ADx NeuroSciences, Ghent, Belgium
Biomarkable, Ghent, Belgium.
Issue Date: 16-Apr-2021
metadata.dc.date: 2021
Publication information: Alzheimer's Research & Therapy 2021; 13(1):79
Abstract: CSF biomarkers are well-established for routine clinical use, yet a paucity of comparative assessment exists regarding CSF extraction methods during lumbar puncture. Here, we compare in detail biomarker profiles in CSF extracted using either gravity drip or aspiration. Biomarkers for β-amyloidopathy (Aβ1-42, Aβ1-40), tauopathy (total tau), or synapse pathology (BACE1, Neurogranin Trunc-p75, α-synuclein) were assessed between gravity or aspiration extraction methods in a sub-population of the Australian Imaging, Biomarkers and Lifestyle (AIBL) study (cognitively normal, N = 36; mild cognitive impairment, N = 8; Alzheimer's disease, N = 6). High biomarker concordance between extraction methods was seen (concordance correlation > 0.85). Passing Bablock regression defined low beta coefficients indicating high scalability. Levels of these commonly assessed CSF biomarkers are not influenced by extraction method. Results of this study should be incorporated into new consensus guidelines for CSF collection, storage, and analysis of biomarkers.
URI: https://ahro.austin.org.au/austinjspui/handle/1/26239
DOI: 10.1186/s13195-021-00812-9
PubMed URL: 33863377
Type: Journal Article
Subjects: Alzheimer’s disease
Amyloid beta
Biomarkers
Cerebrospinal fluid
Collection
Concordance
Appears in Collections:Journal articles

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