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Title: Changes in white adipose tissue gene expression in a randomized control trial of dieting obese men with lowered serum testosterone alone or in combination with testosterone treatment.
Austin Authors: Grossmann, Mathis ;Ng Tang Fui, Mark ;Nie, Tian;Hoermann, Rudolf;Clarke, Michele V;Cheung, Ada S ;Zajac, Jeffrey D ;Davey, Rachel A
Affiliation: Medicine (University of Melbourne)
Issue Date: Aug-2021 2021-04-17
Publication information: Endocrine 2021; 73(2): 463-471
Abstract: The aim of this study was to determine early weight loss-associated changes in subcutaneous abdominal white adipose tissue (WAT) gene expression in obese men with lowered serum testosterone by RNA next-generation sequencing. Fourteen men, mean age (IQR) 51.6 years (43.4-54.5), BMI 38.3 kg/m2 (34.6-40.8) and total testosterone 8.4 nmol/L (7.5-9.5) provided subcutaneous WAT samples at baseline and after 2 weeks of a very low energy diet. Body weight loss was similar in participants receiving testosterone (n = 6), -5.27 kg [95% CI -6.17; -4.26], and placebo (n = 8), -4.57 kg [95% CI -6.10; -3.55], p = 0.86. In placebo-treated men, of the 14,410 genes expressed in subcutaneous WAT, four genes, Angiopoietin-like 4, Semaphorin 3 G, Neuropilin 2 and Angiopoietin 4, were upregulated (adjusted false discovery rate P < 0.05). In an exploratory analysis comparing men receiving testosterone and placebo, the most-upregulated gene in the testosterone group (exploratory p < 0.0005) was the neuropeptide y receptor 2. In obese men, dieting is associated with upregulation of WAT-expressed Angiopoietin-like 4, a secreted protein that regulates lipid metabolism, Semaphorin 3 G, a proposed adipocyte differentiation factor and secreted adipokine, and its receptor Neuropilin 2, as well as Angiopoietin 4, a vascular integrity factor. In an exploratory analysis, testosterone was associated with the upregulation of neuropeptide y receptor 2, a receptor involved in appetite regulation. Further studies are needed to confirm these observations and their potential biological implications., Identifier NCT01616732, Registration date: June 8, 2012.
DOI: 10.1007/s12020-021-02722-0
ORCID: 0000-0001-8261-3457
Journal: Endocrine
PubMed URL: 33864607
Type: Journal Article
Subjects: Gene expression
White adipose tissue
Appears in Collections:Journal articles

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