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Title: Three Dimensional Glomerular Reconstruction: A Novel Approach to Evaluate Renal Microanatomy in Diabetic Kidney Disease.
Austin Authors: Torkamani, Niloufar ;Jerums, George ;Crammer, Paul;Skene, Alison ;Power, David A ;Panagiotopoulos, Sianna ;Clarke, Michele V;MacIsaac, Richard J;Ekinci, Elif I 
Affiliation: Medicine (University of Melbourne)
Department of Endocrinology & Diabetes, St. Vincent's Hospital Melbourne and University of Melbourne, Fitzroy, Victoria, Australia
Department of Anatomical Pathology, Monash Medical Centre, Melbourne, Victoria, Australia
Issue Date: 12-Feb-2019
Date: 2019-02-12
Publication information: Scientific Reports 2019; 9(1): 1829
Abstract: Mesangial metrics reflect glomerular filtration surface area in diabetes. The point-sampled intercept (PSI) method is the conventional method to calculate these parameters. However, this is time consuming and subject to underestimation. We introduce a novel three-dimensional (3D) reconstruction method applicable to light microscopy to measure mesangial metrics. Transmission electron microscopy (TEM), PSI and our new 3D imaging methods were used to quantify mesangial metrics from 22 patients with type 2 diabetes, normo-, micro- and macroalbuminuria and an estimated glomerular filtration rate of <60 mL/min/1.73 m2. Repeated-measures ANOVA test was used to test the equality of the measurement means from the three methods and the degree of inter method variability. Repeated-measures and post-estimation ANOVA tests together with correlation coefficient measurements were used to compare the methods with TEM as reference. There was a statistically significant difference in mesangial volume measurements (F(2, 16) = 15.53, p = 0.0002). The PSI method underestimated measurements compared to TEM and 3D methods by 30% (p = 0.001) and 15%, respectively (p < 0.001). 3D and TEM measurements did not differ significantly. 3D reconstruction is a reliable and time efficient method for calculating mesangial metrics. It may prove to be a useful tool in clinical and experimental diabetic kidney disease.
DOI: 10.1038/s41598-019-38646-z
ORCID: 0000-0001-6259-8675
Journal: Scientific Reports
PubMed URL: 30755701
Type: Journal Article
Appears in Collections:Journal articles

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