Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/20322
Full metadata record
DC FieldValueLanguage
dc.contributor.authorTorkamani, Niloufar-
dc.contributor.authorJerums, George-
dc.contributor.authorCrammer, Paul-
dc.contributor.authorSkene, Alison-
dc.contributor.authorPower, David A-
dc.contributor.authorPanagiotopoulos, Sianna-
dc.contributor.authorClarke, Michele V-
dc.contributor.authorMacIsaac, Richard J-
dc.contributor.authorEkinci, Elif I-
dc.date2019-02-12-
dc.date.accessioned2019-03-04T22:04:16Z-
dc.date.available2019-03-04T22:04:16Z-
dc.date.issued2019-02-12-
dc.identifier.citationScientific Reports 2019; 9(1): 1829en_US
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/20322-
dc.description.abstractMesangial metrics reflect glomerular filtration surface area in diabetes. The point-sampled intercept (PSI) method is the conventional method to calculate these parameters. However, this is time consuming and subject to underestimation. We introduce a novel three-dimensional (3D) reconstruction method applicable to light microscopy to measure mesangial metrics. Transmission electron microscopy (TEM), PSI and our new 3D imaging methods were used to quantify mesangial metrics from 22 patients with type 2 diabetes, normo-, micro- and macroalbuminuria and an estimated glomerular filtration rate of <60 mL/min/1.73 m2. Repeated-measures ANOVA test was used to test the equality of the measurement means from the three methods and the degree of inter method variability. Repeated-measures and post-estimation ANOVA tests together with correlation coefficient measurements were used to compare the methods with TEM as reference. There was a statistically significant difference in mesangial volume measurements (F(2, 16) = 15.53, p = 0.0002). The PSI method underestimated measurements compared to TEM and 3D methods by 30% (p = 0.001) and 15%, respectively (p < 0.001). 3D and TEM measurements did not differ significantly. 3D reconstruction is a reliable and time efficient method for calculating mesangial metrics. It may prove to be a useful tool in clinical and experimental diabetic kidney disease.en_US
dc.language.isoeng-
dc.titleThree Dimensional Glomerular Reconstruction: A Novel Approach to Evaluate Renal Microanatomy in Diabetic Kidney Disease.en_US
dc.typeJournal Articleen_US
dc.identifier.journaltitleScientific Reportsen_US
dc.identifier.affiliationMedicine (University of Melbourne)en_US
dc.identifier.affiliationEndocrinologyen_US
dc.identifier.affiliationDepartment of Endocrinology & Diabetes, St. Vincent's Hospital Melbourne and University of Melbourne, Fitzroy, Victoria, Australiaen_US
dc.identifier.affiliationDepartment of Anatomical Pathology, Monash Medical Centre, Melbourne, Victoria, Australiaen_US
dc.identifier.affiliationNephrologyen_US
dc.identifier.doi10.1038/s41598-019-38646-zen_US
dc.type.contentTexten_US
dc.identifier.orcid0000-0001-6259-8675en_US
dc.identifier.orcid0000-0003-3983-0581en_US
dc.identifier.orcid0000-0002-0845-0001en_US
dc.identifier.orcid0000-0003-2372-395Xen_US
dc.identifier.pubmedid30755701-
dc.type.austinJournal Article-
local.name.researcherEkinci, Elif I
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptEndocrinology-
crisitem.author.deptEndocrinology-
crisitem.author.deptPathology-
crisitem.author.deptMedicine (University of Melbourne)-
crisitem.author.deptInstitute for Breathing and Sleep-
crisitem.author.deptOffice for Research-
crisitem.author.deptEndocrinology-
Appears in Collections:Journal articles
Show simple item record

Page view(s)

44
checked on Mar 28, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.