A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling.

2016-10-19

Verberne, Anthony J M

Picaxe

juxtacellular

microcontroller

neuron

neurophysiology

Journal Article

0000-0002-2049-1439

10.3390/bioengineering3040027

Juxtacellular neuronal labelling is a method which allows neurophysiologists to fill physiologically-identified neurons with small positively-charged marker molecules. Labelled neurons are identified by histochemical processing of brain sections along with immunohistochemical identification of neuropeptides, neurotransmitters, neurotransmitter transporters or biosynthetic enzymes. A microcontroller-based pulser circuit and associated BASIC software script is described for incorporation into the design of a commercially-available intracellular electrometer for use in juxtacellular neuronal labelling. Printed circuit board construction has been used for reliability and reproducibility. The current design obviates the need for a separate digital pulse source and simplifies the juxtacellular neuronal labelling procedure.

link

Bioengineering (Basel, Switzerland) 2016; 3(4): E27

Bioengineering (Basel, Switzerland)

2306-5354