Please use this identifier to cite or link to this item:
Title: Spontaneous T cell responses to melanoma differentiation antigens from melanoma patients and healthy subjects.
Austin Authors: Chen, Q;Jackson, Heather M;Gibbs, P;Davis, Ian D;Trapani, Joseph A;Cebon, Jonathan S 
Affiliation: Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Austin Repat Cancer Centre, Heidelberg, Victoria, Australia
Issue Date: 1-Dec-1998
Publication information: Cancer Immunology, Immunotherapy : Cii; 47(4): 191-7
Abstract: The spontaneous cytotoxic T cell responses to melanoma differentiation antigens and influenza matrix peptide were compared in 20 HLA-A2+ melanoma patients and 17 healthy A2+ individuals. Cytotoxic T lymphocyte (CTL) responses were determined by mixed lymphocyte peptide culture (MLPC) involving two stimulations of unfractionated peripheral blood lymphocytes (PBLs) with peptide in vitro. CTL responses to Melan-A 9-mer (amino acids 27-35, AAGIGILTV) peptide were detected in 4 out of 16 normal individuals, but in none of the melanoma patients. CTL specific for influenza matrix peptide were frequently found in both normal individuals and melanoma patients, suggesting that generalized immuno-suppression was not responsible for this difference. No significant responses were observed in either normal individuals or melanoma patients to Melan-A 10-mer (26-35, EAAGIGILTV), two gp1OO epitopes (280-288, YLEPGPVTA; 457466, LLDGTATLRL) and two tyrosinase epitopes (1-9, MLLAVLYCL; 368-376, YMDGMSQV). Melan-A (27-35)-specific CTL cells generated by normal individuals and melanoma patients recognized both synthetic peptide-pulsed T2 cells and two HLA-A2+, Melan-A+ melanoma cell lines (ME272, LAR1) in an antigen-specific, MHC class I restricted manner. T cells generated against Melan-A 9-mer were also able to recognize Melan-A 10-mer-pulsed target cells. Spontaneous CTL responses to Melan-A 9-mer from three known responder normal individuals were further evaluated over a prolonged time course (6-11 months). All 3 subjects demonstrated specific Melan-A 9-mer responses throughout the study period, although lytic activity fluctuated over time for a given individual. We found the MLPC assay to be reliable and easy to perform for monitoring T cell responses, although it may still not be sufficiently sensitive to detect low numbers of precursor T cells.
Gov't Doc #: 9875671
Journal: Cancer immunology, immunotherapy : CII
Type: Journal Article
Subjects: Adjuvants, Immunologic.pharmacology
Amino Acid Sequence
Antigens, Differentiation.immunology.pharmacology
Antigens, Neoplasm
Lymphocyte Activation.drug effects
MART-1 Antigen
Neoplasm Proteins.immunology.pharmacology
T-Lymphocytes, Cytotoxic.drug effects.immunology
Appears in Collections:Journal articles

Show full item record

Page view(s)

checked on Dec 10, 2023

Google ScholarTM


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.