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dc.contributor.authorChen, Qen
dc.contributor.authorJackson, Heather Men
dc.contributor.authorGibbs, Pen
dc.contributor.authorDavis, Ian Den
dc.contributor.authorTrapani, Joseph Aen
dc.contributor.authorCebon, Jonathan Sen
dc.identifier.citationCancer Immunology, Immunotherapy : Cii; 47(4): 191-7en
dc.description.abstractThe spontaneous cytotoxic T cell responses to melanoma differentiation antigens and influenza matrix peptide were compared in 20 HLA-A2+ melanoma patients and 17 healthy A2+ individuals. Cytotoxic T lymphocyte (CTL) responses were determined by mixed lymphocyte peptide culture (MLPC) involving two stimulations of unfractionated peripheral blood lymphocytes (PBLs) with peptide in vitro. CTL responses to Melan-A 9-mer (amino acids 27-35, AAGIGILTV) peptide were detected in 4 out of 16 normal individuals, but in none of the melanoma patients. CTL specific for influenza matrix peptide were frequently found in both normal individuals and melanoma patients, suggesting that generalized immuno-suppression was not responsible for this difference. No significant responses were observed in either normal individuals or melanoma patients to Melan-A 10-mer (26-35, EAAGIGILTV), two gp1OO epitopes (280-288, YLEPGPVTA; 457466, LLDGTATLRL) and two tyrosinase epitopes (1-9, MLLAVLYCL; 368-376, YMDGMSQV). Melan-A (27-35)-specific CTL cells generated by normal individuals and melanoma patients recognized both synthetic peptide-pulsed T2 cells and two HLA-A2+, Melan-A+ melanoma cell lines (ME272, LAR1) in an antigen-specific, MHC class I restricted manner. T cells generated against Melan-A 9-mer were also able to recognize Melan-A 10-mer-pulsed target cells. Spontaneous CTL responses to Melan-A 9-mer from three known responder normal individuals were further evaluated over a prolonged time course (6-11 months). All 3 subjects demonstrated specific Melan-A 9-mer responses throughout the study period, although lytic activity fluctuated over time for a given individual. We found the MLPC assay to be reliable and easy to perform for monitoring T cell responses, although it may still not be sufficiently sensitive to detect low numbers of precursor T cells.en
dc.subject.otherAdjuvants, Immunologic.pharmacologyen
dc.subject.otherAmino Acid Sequenceen
dc.subject.otherAntigens, Differentiation.immunology.pharmacologyen
dc.subject.otherAntigens, Neoplasmen
dc.subject.otherLymphocyte Activation.drug effectsen
dc.subject.otherMART-1 Antigenen
dc.subject.otherNeoplasm Proteins.immunology.pharmacologyen
dc.subject.otherT-Lymphocytes, Cytotoxic.drug effects.immunologyen
dc.titleSpontaneous T cell responses to melanoma differentiation antigens from melanoma patients and healthy subjects.en
dc.typeJournal Articleen
dc.identifier.journaltitleCancer immunology, immunotherapy : CIIen
dc.identifier.affiliationLudwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Austin Repat Cancer Centre, Heidelberg, Victoria, Australiaen
dc.type.austinJournal Articleen, Jonathan S
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.grantfulltextnone- Newton-John Cancer Research Institute-
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