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Title: Amylin as a growth factor during fetal and postnatal development of the rat kidney.
Austin Authors: Wookey, Peter J ;Tikellis, Christos;Nobes, M;Casley, David J;Cooper, Mark E;Darby, I A
Affiliation: Department of Medicine, University of Melbourne, Bundoora, Victoria, Australia
Issue Date: 1-Jan-1998
Publication information: Kidney International; 53(1): 25-30
Abstract: We have previously reported that amylin has mitogenic actions on tubular epithelial cells isolated from mature rat kidney and cultured in vitro. In experiments using in situ hybridization, we have demonstrated that amylin mRNA can be detected transiently in rat metanephros from embryo day 17 (E17) to postnatal day 3 (PN3). These transcripts are localized in the sub-nephrogenic zone. RT-PCR was performed using oligonucleotide primers for rat amylin and mRNA extracted from fetal body (E19), PN1 and PN5 metanephroi, and adult rat kidney. These results corroborate the finding, using in situ hybridization, that there is a window of expression of rat amylin in the developing kidney in the perinatal period. During this period tubular elongation is evident and amylin peptide, detected by immunohistochemical staining, is found associated with developing tubules. Some of these tubules also express a brush border glycoprotein, detected by immunohistochemical staining. Amylin acts as a mitogen with primary cultures of proximal tubular epithelial cells from PN4 renal cortex. An amylin antagonist inhibited this mitogenic action suggesting that this was mediated by amylin receptors as previously described. We suggest that amylin peptide is biosynthesized in the developing proximal tubules, acts in an autocrine fashion to promote the proliferation and differentiation of brush border epithelial cells and hence plays an important role as a growth factor in the development of the kidney.
Gov't Doc #: 9452996
DOI: 10.1046/j.1523-1755.1998.00722.x
Type: Journal Article
Subjects: Amino Acid Sequence
Cells, Cultured
Growth Substances.physiology
Islet Amyloid Polypeptide
Kidney.embryology.growth & development
Molecular Sequence Data
RNA, Messenger.analysis
Rats, Sprague-Dawley
Appears in Collections:Journal articles

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