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Title: Interaction between epidermal growth factor and arginine vasopressin in renal medullary membranes.
Austin Authors: Grant, Sharon L;Phillips, P A;Gow, Paul J 
Affiliation: Department of Medicine, University of Melbourne, Austin Hospital, Victoria, Australia
Issue Date: 1-Mar-1994
Publication information: Clinical and Experimental Pharmacology & Physiology; 21(3): 243-7
Abstract: 1. Epidermal growth factor is a potent mitogen that causes natriuresis, diuresis and inhibition of arginine vasopressin-induced water reabsorption. 2. The aim of this study was to determine any interaction between epidermal growth factor and the V1 (vascular) and/or V2 (antidiuretic) arginine vasopressin receptor subtypes. 3. Radioligand binding displacement assays demonstrated that although arginine vasopressin related peptides displaced both radioligands from renal medullary membranes at low concentrations epidermal growth factor displaced neither. 4. Arginine vasopressin V2 receptor second messenger cyclic adenosine monophosphate (cAMP) production was inhibited by epidermal growth factor (IC50 2 x 10(-7) mol/L) as was sodium fluoride cAMP production but only at much higher concentrations. 5. Therefore the diuretic effect of epidermal growth factor is not via direct antagonism of arginine vasopressin receptors but seems mediated via inhibition of the V2 second messenger system.
Gov't Doc #: 8076430
Journal: Clinical and Experimental Pharmacology & Physiology
Type: Journal Article
Subjects: Adenylate Cyclase.metabolism.physiology
Angiotensin II.pharmacology
Arginine Vasopressin.antagonists & inhibitors.metabolism.pharmacology
Binding, Competitive
Cyclic AMP.biosynthesis
Drug Interactions
Epidermal Growth Factor.physiology
Kidney Medulla.drug effects.metabolism.physiology
Membranes.drug effects.metabolism
Radioligand Assay
Rats, Sprague-Dawley
Receptors, Vasopressin.metabolism.physiology
Second Messenger Systems.drug effects.physiology
Appears in Collections:Journal articles

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