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Title: Induction of Ca2+ signaling and possible exocytosis in endothelial cells by a stable leukocyte-derived factor.
Austin Authors: Liu, J J;Chen, J R;Bradley, C J;Johnston, Colin I;Buxton, Brian F 
Affiliation: University of Melbourne, Austin Hospital, Department of Cardiac Surgery, Heidelberg, Victoria, Australia
Issue Date: 5-Jan-1995
Publication information: Biochemical and Biophysical Research Communications; 206(1): 146-52
Abstract: Previous studies have shown that polymorphonuclear leukocytes (PMNs) release a stable factor that inhibits endothelium-dependent relaxation. In the present studies, the effects of the factor on Ca2+ signaling and on ultrastructure of endothelial cells were investigated. In the cultured endothelial cells, the PMN-derived factor induced an increase in [Ca2+]i in a pattern of oscillations. The frequency of the Ca2+ oscillations was less than 3 spikes/10 minutes. Removal of extracellular Ca2+ by perfusion with Ca(2+)-free Krebs' solution abolished the spikes. The results of electron microscopy showed that this factor induced an increase in vesicle formation on the luminal surface of the rat aortic endothelium. The increased vesicle formation may represent exocytosis. The structure of the smooth muscle cells was not changed. In conclusion, the PMN-derived factor induces a Ca2+ influx and In conclusion, the PMN-derived factor induces a Ca2+ influx and possible exocytosis, suggesting that the factor may have other biological functions besides the inhibition of the vascular relaxation.
Gov't Doc #: 7818514
DOI: 10.1006/bbrc.1995.1021
Type: Journal Article
Subjects: Animals
Biological Factors.isolation & purification.pharmacology
Cells, Cultured
Endothelium, Vascular.drug effects.physiology.ultrastructure
Exocytosis.drug effects
In Vitro Techniques
Microscopy, Electron
Signal Transduction.drug effects
Time Factors
Appears in Collections:Journal articles

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