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Title: Characterisation of angiotensin converting enzyme from different rat vascular beds.
Austin Authors: Jandeleit-Dahm, K;Jackson, B ;Paxton, D;Perich, R;Johnston, Colin I
Affiliation: Medicine (University of Melbourne)
Issue Date: 1-May-1995
Publication information: Blood Pressure; 4(3): 170-6
Abstract: The tissue renin angiotensin system may play a role in cardiovascular pathophysiology. Angiotensin converting enzyme in tissues is now a target for pharmacological inhibition. It is therefore important to determine whether ACE is evenly distributed throughout the vascular tree and whether the enzyme has the same characteristics in different vascular beds. We have thus measured angiotensin converting enzyme density in three functionally different vascular beds with three different methods: the enzyme kinetic assay, a radioligand binding assay and in vitro autoradiography. All three methods demonstrated a significantly higher binding density and activity of ACE in resistance arteries from the mesenteric vascular bed of rats than in microvessels from the brain, or in a conduit artery, the aorta. The dissociation constant (Kd) of the enzyme-radioligand complex was the same in the three functionally different vessel types. Radioligand displacement studies for ACE from plasma and the mesenteric vessels in vitro utilizing a panel of different ACE inhibitors have shown a similar rank order of inhibitory potency suggesting that catalytic sites of ACE were the same in plasma and the mesenteric microvessels. In vivo, the enzyme inhibition in plasma, mesenteric and brain vessels measured by enzyme kinetic and radioligand binding assay were well correlated. There was a similar degree of inhibition between different vessels and tissues (mesenteric vessels, aorta, kidney, left ventricle and coronaries) measured by in vitro autoradiography.
Journal: Blood pressure
Type: Journal Article
Subjects: Angiotensin-Converting Enzyme Inhibitors.pharmacology
Aorta, Thoracic.enzymology
Bicyclo Compounds.pharmacology
Bicyclo Compounds, Heterocyclic
Binding Sites
Brain.blood supply
Mesenteric Arteries.enzymology
Peptidyl-Dipeptidase A.analysis
Rats, Inbred WKY
Rats, Sprague-Dawley
Appears in Collections:Journal articles

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