Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13072
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dc.contributor.authorJandeleit-Dahm, K-
dc.contributor.authorJackson, B-
dc.contributor.authorPaxton, D-
dc.contributor.authorPerich, R-
dc.contributor.authorJohnston, Colin I-
dc.date.accessioned2015-05-16T02:51:05Z
dc.date.available2015-05-16T02:51:05Z
dc.date.issued1995-05-01-
dc.identifier.citationBlood Pressure; 4(3): 170-6en_US
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/13072en
dc.description.abstractThe tissue renin angiotensin system may play a role in cardiovascular pathophysiology. Angiotensin converting enzyme in tissues is now a target for pharmacological inhibition. It is therefore important to determine whether ACE is evenly distributed throughout the vascular tree and whether the enzyme has the same characteristics in different vascular beds. We have thus measured angiotensin converting enzyme density in three functionally different vascular beds with three different methods: the enzyme kinetic assay, a radioligand binding assay and in vitro autoradiography. All three methods demonstrated a significantly higher binding density and activity of ACE in resistance arteries from the mesenteric vascular bed of rats than in microvessels from the brain, or in a conduit artery, the aorta. The dissociation constant (Kd) of the enzyme-radioligand complex was the same in the three functionally different vessel types. Radioligand displacement studies for ACE from plasma and the mesenteric vessels in vitro utilizing a panel of different ACE inhibitors have shown a similar rank order of inhibitory potency suggesting that catalytic sites of ACE were the same in plasma and the mesenteric microvessels. In vivo, the enzyme inhibition in plasma, mesenteric and brain vessels measured by enzyme kinetic and radioligand binding assay were well correlated. There was a similar degree of inhibition between different vessels and tissues (mesenteric vessels, aorta, kidney, left ventricle and coronaries) measured by in vitro autoradiography.en_US
dc.language.isoenen
dc.subject.otherAngiotensin-Converting Enzyme Inhibitors.pharmacologyen
dc.subject.otherAnimalsen
dc.subject.otherAorta, Thoracic.enzymologyen
dc.subject.otherBicyclo Compounds.pharmacologyen
dc.subject.otherBicyclo Compounds, Heterocyclicen
dc.subject.otherBinding Sitesen
dc.subject.otherBrain.blood supplyen
dc.subject.otherCatalysisen
dc.subject.otherMaleen
dc.subject.otherMesenteric Arteries.enzymologyen
dc.subject.otherPeptidyl-Dipeptidase A.analysisen
dc.subject.otherRatsen
dc.subject.otherRats, Inbred WKYen
dc.subject.otherRats, Sprague-Dawleyen
dc.titleCharacterisation of angiotensin converting enzyme from different rat vascular beds.en_US
dc.typeJournal Articleen_US
dc.identifier.journaltitleBlood pressureen_US
dc.identifier.affiliationMedicine (University of Melbourne)en_US
dc.description.pages170-6en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/7670651en
dc.type.contentTexten_US
dc.type.austinJournal Articleen
local.name.researcherJackson, Belinda D
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptGastroenterology and Hepatology-
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