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Title: Disparate effects of castration on renal structure and function in the streptozotocin diabetic rat.
Austin Authors: Bach, Leon A;Cooper, Mark E;Vranes, D;Allen, Terri J;Rumble, J R;Jerums, George 
Affiliation: Endocrine Unit, Austin Hospital, Heidelberg, Victoria, Australia
Issue Date: 16-May-1994
Publication information: Diabetes Research (edinburgh, Scotland); 27(1): 27-38
Abstract: Diabetic children are protected from the complications of diabetes until puberty, suggesting the involvement of a hormonal mechanism. In order to investigate the contribution of sex steroids to the development of diabetic nephropathy, castrated Wistar-Kyoto rats were made diabetic with streptozotocin and compared with intact diabetic rats. In intact rats, 32 weeks of diabetes resulted in hyperfiltration (p = 0.0001) and a marked increase in albuminuria (control, 1.3 x/divided by 1.3 vs diabetes intact, 11.8 x/divided by 1.3 mg/day, p < 0.001). Concomitant structural changes included glomerular basement membrane thickening (diabetes, 199.2 +/- 7.9 vs control, 175.9 +/- 3.9 nm, p = 0.02) and increased mesangial volume (diabetes, 194 +/- 14 vs control, 129 +/- 20 microns 3, p = 0.02). Although castration resulted in blood pressure lowering of 12 mmHg (p = 0.0003), albuminuria increased to the same extent in intact and castrated diabetic rats (castrated diabetic 10.5 x/divided by 1.3 mg/day). Glomerular filtration rate was lower in castrated rats than in intact rats prior to induction of diabetes, but increased similarly with induction of diabetes. Castrated diabetic rats had increased mesangial volume (castrated diabetic, 213 +/- 20 microns 3), but glomerular basement membrane thickness was lower than in intact diabetic rats (castrated diabetic, 179.5 +/- 4.5 nm). Thus, there is a dissociation between basement membrane thickening and albuminuria in this model of diabetic kidney disease. The mechanism of action of castration on glomerular function and structure remains to be elucidated but may relate to indirect effects such as reduction of blood pressure or renal polyol accumulation or a direct effect of sex steroids.
Gov't Doc #: 7648794
Type: Journal Article
Subjects: Albuminuria
Basement Membrane.pathology
Blood Glucose.metabolism
Blood Pressure
Body Weight
Diabetes Mellitus, Experimental.pathology.physiopathology
Glomerular Filtration Rate
Glomerular Mesangium.cytology.pathology
Kidney Glomerulus.pathology
Rats, Inbred WKY
Reference Values
Time Factors
Appears in Collections:Journal articles

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