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Title: Non-invasive assessment of the efficacy of new therapeutics for intestinal pathologies using serial endoscopic imaging of live mice
Austin Authors: Ernst, Matthias ;Preaudet, Adele;Putoczki, Tracy
Affiliation: Olivia Newton-John Cancer Research Institute, Heidelberg, Victoria, Australia
The Walter and Eliza Hall Institute for Medical Research, Parkville, Victoria, Australia
The Department of Medical Biology, University of Melbourne, Victoria, Australia
Issue Date: 10-Mar-2015
Publication information: Journal of Visualized Experiments; 2015; (97): e52383
Abstract: Animal models of inflammatory bowel disease (IBD) and colorectal cancer (CRC) have provided significant insight into the cell intrinsic and extrinsic mechanisms that contribute to the onset and progression of intestinal diseases. The identification of new molecules that promote these pathologies has led to a flurry of activity focused on the development of potential new therapies to inhibit their function. As a result, various pre-clinical mouse models with an intact immune system and stromal microenvironment are now heavily used. Here we describe three experimental protocols to test the efficacy of new therapeutics in pre-clinical models of (1) acute mucosal damage, (2) chronic colitis and/or colitis-associated colon cancer, and (3) sporadic colorectal cancer. We also outline procedures for serial endoscopic examination that can be used to document the therapeutic response of an individual tumor and to monitor the health of individual mice. These protocols provide complementary experimental platforms to test the effectiveness of therapeutic compounds shown to be well tolerated by mice.
Gov't Doc #: 25867916
DOI: 10.3791/52383
Journal: Journal of Visualized Experiments
Type: Journal Article
Appears in Collections:Journal articles

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