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Title: A novel method for detecting antigen-specific human regulatory T cells.
Austin Authors: Ebert, Lisa M;MacRaild, Sarah E;Davis, Ian D;Cebon, Jonathan S ;Chen, Weisan
Affiliation: Ludwig Institute for Cancer Research (Melbourne-Austin Branch), Melbourne, Australia
Issue Date: 13-Jan-2012
Publication information: Journal of Immunological Methods 2012; 377(1-2): 56-61
Abstract: Antigenic epitopes recognized by FoxP3(+) regulatory T cells (Treg) are poorly defined, largely due to a lack of assays for determining Treg specificity. We have developed a novel approach for detecting human Treg specific to peptide antigen, utilizing down-regulation of surface CD3 as a read-out of antigen recognition. Culture conditions and re-stimulation time have been optimized, allowing the detection of even very rare Treg, such as those specific to tumor antigens.
Gov't Doc #: 22265970
DOI: 10.1016/j.jim.2012.01.004
Journal: Journal of immunological methods
Type: Journal Article
Subjects: Antigen Presentation.immunology
Antigens, CD3.genetics.immunology
Epitopes, T-Lymphocyte.immunology
Flow Cytometry.methods
Forkhead Transcription Factors.immunology
T-Lymphocytes, Regulatory.immunology
Appears in Collections:Journal articles

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