A novel method for detecting antigen-specific human regulatory T cells.

2012-01-13

Ebert, Lisa M

MacRaild, Sarah E

Davis, Ian D

Cebon, Jonathan S

Chen, Weisan

Journal Article

10.1016/j.jim.2012.01.004

Antigenic epitopes recognized by FoxP3(+) regulatory T cells (Treg) are poorly defined, largely due to a lack of assays for determining Treg specificity. We have developed a novel approach for detecting human Treg specific to peptide antigen, utilizing down-regulation of surface CD3 as a read-out of antigen recognition. Culture conditions and re-stimulation time have been optimized, allowing the detection of even very rare Treg, such as those specific to tumor antigens.

link

Journal of Immunological Methods 2012; 377(1-2): 56-61

Journal of immunological methods