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Title: Recognition of a carbohydrate xenoepitope by human NKRP1A (CD161).
Austin Authors: Christiansen, Dale;Mouhtouris, Effie ;Milland, Julie;Zingoni, Alessandra;Santoni, Angela;Sandrin, Mauro S 
Affiliation: Surgery
Issue Date: 1-Sep-2006
Publication information: Xenotransplantation; 13(5): 440-6
Abstract: Many immunologically important interactions are mediated by leukocyte recognition of carbohydrates via cell surface receptors. Uncharacterized receptors on human natural killer (NK) cells interact with ligands containing the terminal Galalpha(1,3)Gal xenoepitope. The aim of this work was to isolate and characterize carbohydrate binding proteins from NK cells that bind alphaGal or other potential xenoepitopes, such as N-acetyllactosamine (NAcLac), created by the deletion of alpha1,3galactosyltransferase (GT) in animals.Initial analysis suggested the human C-type lectin NKRP1A bound to a pool of glycoconjugates, the majority of which contained the terminal Galalpha(1,3)Gal epitope. This was confirmed by high level binding of cells expressing NKRP1A to mouse laminin, which contains a large number of N-linked oligosaccharides with the Galalpha(1,3)Gal structure. The consequence of removing the terminal alphaGal was then investigated. Elevated NAcLac levels were observed on thymocytes from GT-/- mice. Exposing NAcLac on laminin, by alpha-galactosidase treatment, resulted in a significant increase in NKRP1A binding.NKRPIA binds to the alphaGal epitope. Moreover, exposing NAcLac by removal of alphaGal resulted in an increase in binding. This may be relevant in the later phases of xenotransplant rejection if GT-/- pigs, like GT-/- mice, display increased NAcLac expression.
DOI: 10.1111/j.1399-3089.2006.00332.x
Journal: Xenotransplantation
Type: Journal Article
Subjects: Amino Sugars.biosynthesis.immunology
Antigen-Antibody Reactions
Antigens, Surface.immunology
COS Cells
Cercopithecus aethiops
Lectins, C-Type.immunology
NK Cell Lectin-Like Receptor Subfamily B
Transplantation, Heterologous.immunology
Appears in Collections:Journal articles

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