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Title: Differential effect of inbred mouse strain (C57BL/6, DBA/2, 129T2) on insulin secretory function in response to a high fat diet.
Austin Authors: Andrikopoulos, Sofianos;Massa, Christine M;Aston-Mourney, Kathryn;Funkat, Alexandra;Fam, Barbara C;Hull, Rebecca L;Kahn, Steven E;Proietto, Joseph 
Affiliation: University of Melbourne, Department of Medicine, Heidelberg Repatriation Hospital, Heidelberg Heights, Victoria 3081, Australia
Issue Date: 1-Oct-2005
Publication information: The Journal of Endocrinology; 187(1): 45-53
Abstract: The increasing production of genetically-modified mouse models has necessitated studies to determine the inherent physiological characteristics of commonly used mouse strains. In this study we examined insulin secretory function in response to an intravenous bolus of glucose or glucose plus arginine in anesthetized C57BL/6, DBA/2 and 129T2 mice fed either a control or high fat diet for 6 weeks. The results show that 129T2 mice had higher fasting plasma glucose levels and lower fasting plasma insulin levels compared with C57BL/6 and DBA/2 mice regardless of diet. Furthermore, 129T2 mice were glucose intolerant and secreted significantly less insulin in response to glucose and glucose plus arginine irrespective of diet compared with the other two strains of mice. DBA/2 mice hypersecreted insulin in response to glucose and glucose plus arginine compared with C57BL/6 and 129T2 mice. Moreover while first phase insulin secretion was appropriately increased in response to the high fat diet in C57BL/6 and 129T2 mice, this was not the case for DBA/2 mice. Mean islet area was decreased in response to a high fat diet in DBA/2 mice, while there was no dietary effect on the other two strains. This study highlights the inherent genetic differences that exist among seemingly normal strains of mice that are commonly used to make transgenic and knockout mice. Understanding these differences will provide researchers with the information to choose the appropriate genetic background on which to express their particular genetic alteration.
Gov't Doc #: 16214940
DOI: 10.1677/joe.1.06333
Type: Journal Article
Subjects: Animals
Arginine.diagnostic use
Dietary Fats.administration & dosage
Glucose.diagnostic use
Islets of Langerhans.cytology.drug effects.secretion
Mice, Inbred C57BL
Mice, Inbred DBA
Mice, Inbred Strains.physiology
Species Specificity
Appears in Collections:Journal articles

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