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Title: | Insulin-like growth factor (IGF)-binding protein-6 inhibits IGF-II-induced but not basal proliferation and adhesion of LIM 1215 colon cancer cells. | Austin Authors: | Leng, S L;Leeding, K S;Whitehead, R H;Bach, Leon A | Affiliation: | Department of Medicine, University of Melbourne, Austin and Repatriation Medical Centre, Heidelberg, Vic. 3084, Australia | Issue Date: | 28-Mar-2001 | Publication information: | Molecular and Cellular Endocrinology; 174(1-2): 121-7 | Abstract: | IGF-II is an autocrine growth factor for many colon cancer cells. This study aimed to determine the role of IGF-II in proliferation and adhesion of LIM 1215 colon cancer cells. RT-PCR demonstrated expression of IGF-I and IGF-II mRNA. Addition of IGF-I or -II increased monolayer proliferation in a dose-dependent manner. Although addition of IGFBP-6 had no effect on basal proliferation, coincubation of IGFBP-6 decreased IGF-II but not IGF-I-induced proliferation. Colony formation in agar was increased by IGF-II, an effect inhibited by coincubation with IGFBP-6. IGFBP-6 alone significantly decreased colony formation. Preincubation of cells with IGF-II increased adhesion to type IV collagen, fibronectin and laminin. IGFBP-6 had no effect on basal cell adhesion but completely inhibited the effects of IGF-II. LIM 1215 colon cancer cells are therefore IGF-responsive but IGF-II is not a major autocrine factor for these cells in monolayer, suggesting heterogeneity between colon carcinoma cell lines with respect to the role of the IGF system. | Gov't Doc #: | 11306178 | URI: | https://ahro.austin.org.au/austinjspui/handle/1/9306 | Journal: | Molecular and cellular endocrinology | URL: | https://pubmed.ncbi.nlm.nih.gov/11306178 | Type: | Journal Article | Subjects: | Autocrine Communication Cell Adhesion.drug effects Cell Division.drug effects Colonic Neoplasms.pathology Extracellular Matrix.metabolism Humans Insulin-Like Growth Factor Binding Protein 6.pharmacology Insulin-Like Growth Factor I.genetics Insulin-Like Growth Factor II.antagonists & inhibitors.genetics RNA, Messenger.metabolism Tumor Cells, Cultured |
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