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Title: Insulin-like growth factor (IGF)-binding protein-6 inhibits IGF-II-induced but not basal proliferation and adhesion of LIM 1215 colon cancer cells.
Austin Authors: Leng, S L;Leeding, K S;Whitehead, R H;Bach, Leon A
Affiliation: Department of Medicine, University of Melbourne, Austin and Repatriation Medical Centre, Heidelberg, Vic. 3084, Australia
Issue Date: 28-Mar-2001
Publication information: Molecular and Cellular Endocrinology; 174(1-2): 121-7
Abstract: IGF-II is an autocrine growth factor for many colon cancer cells. This study aimed to determine the role of IGF-II in proliferation and adhesion of LIM 1215 colon cancer cells. RT-PCR demonstrated expression of IGF-I and IGF-II mRNA. Addition of IGF-I or -II increased monolayer proliferation in a dose-dependent manner. Although addition of IGFBP-6 had no effect on basal proliferation, coincubation of IGFBP-6 decreased IGF-II but not IGF-I-induced proliferation. Colony formation in agar was increased by IGF-II, an effect inhibited by coincubation with IGFBP-6. IGFBP-6 alone significantly decreased colony formation. Preincubation of cells with IGF-II increased adhesion to type IV collagen, fibronectin and laminin. IGFBP-6 had no effect on basal cell adhesion but completely inhibited the effects of IGF-II. LIM 1215 colon cancer cells are therefore IGF-responsive but IGF-II is not a major autocrine factor for these cells in monolayer, suggesting heterogeneity between colon carcinoma cell lines with respect to the role of the IGF system.
Gov't Doc #: 11306178
Journal: Molecular and cellular endocrinology
Type: Journal Article
Subjects: Autocrine Communication
Cell Adhesion.drug effects
Cell Division.drug effects
Colonic Neoplasms.pathology
Extracellular Matrix.metabolism
Insulin-Like Growth Factor Binding Protein 6.pharmacology
Insulin-Like Growth Factor I.genetics
Insulin-Like Growth Factor II.antagonists & inhibitors.genetics
RNA, Messenger.metabolism
Tumor Cells, Cultured
Appears in Collections:Journal articles

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