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|Title:||A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling.|
|Authors:||Verberne, Anthony J M|
|Affiliation:||Clinical Pharmacology and Therapeutics Unit, Department of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia|
|Citation:||Bioengineering (Basel, Switzerland) 2016; 3(4): E27|
|Abstract:||Juxtacellular neuronal labelling is a method which allows neurophysiologists to fill physiologically-identified neurons with small positively-charged marker molecules. Labelled neurons are identified by histochemical processing of brain sections along with immunohistochemical identification of neuropeptides, neurotransmitters, neurotransmitter transporters or biosynthetic enzymes. A microcontroller-based pulser circuit and associated BASIC software script is described for incorporation into the design of a commercially-available intracellular electrometer for use in juxtacellular neuronal labelling. Printed circuit board construction has been used for reliability and reproducibility. The current design obviates the need for a separate digital pulse source and simplifies the juxtacellular neuronal labelling procedure.|
|Appears in Collections:||Journal articles|
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