Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/18423
Title: A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling.
Authors: Verberne, Anthony J M
Affiliation: Clinical Pharmacology and Therapeutics Unit, Department of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia
Issue Date: 19-Oct-2016
EDate: 2016-10-19
Citation: Bioengineering (Basel, Switzerland) 2016; 3(4): E27
Abstract: Juxtacellular neuronal labelling is a method which allows neurophysiologists to fill physiologically-identified neurons with small positively-charged marker molecules. Labelled neurons are identified by histochemical processing of brain sections along with immunohistochemical identification of neuropeptides, neurotransmitters, neurotransmitter transporters or biosynthetic enzymes. A microcontroller-based pulser circuit and associated BASIC software script is described for incorporation into the design of a commercially-available intracellular electrometer for use in juxtacellular neuronal labelling. Printed circuit board construction has been used for reliability and reproducibility. The current design obviates the need for a separate digital pulse source and simplifies the juxtacellular neuronal labelling procedure.
URI: http://ahro.austin.org.au/austinjspui/handle/1/18423
DOI: 10.3390/bioengineering3040027
ORCID: 0000-0002-2049-1439
PubMed URL: 28952589
ISSN: 2306-5354
Type: Journal Article
Subjects: Picaxe
juxtacellular
microcontroller
neuron
neurophysiology
Appears in Collections:Journal articles

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