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Title: Recombinant NY-ESO-1 cancer antigen: production and purification under cGMP conditions.
Austin Authors: Murphy, R;Green, S;Ritter, G;Cohen, L;Ryan, D;Woods, W;Rubira, M;Cebon, Jonathan S ;Davis, Ian D;Sjolander, A;Kypridis, A;Kalnins, Renate M;McNamara, M;Moloney, M B;Ackland, J;Cartwright, Glenn A;Rood, J;Dumsday, G;Healey, K;Maher, D;Maraskovsky, Eugene;Chen, Y T;Hoffman, E W;Old, Lloyd J;Scott, Andrew M 
Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Austin Hospital, Heidelberg, Australia
Issue Date: 2005
Publication information: Preparative Biochemistry & Biotechnology; 35(2): 119-34
Abstract: The cancer-testis antigen, NY-ESO-1, has been engineered into a bacterial expression plasmid which incorporates a His6-tag. The plasmid was transfected into E. coli strain BL21 and Master and Working cell banks generated from this expression system. Three 15-litre fermentations were performed under cGMP (code of Good Manufacturing Practice) conditions and the crude NY-ESO-1 tagged protein isolated as solubilised inclusion bodies. A three-step cGMP chromatography process (immobilised metal affinity, anion exchange, and hydrophobic interaction) was utilised to purify the protein. The purified NY-ESO-1 is being used in early stage human cancer vaccine trials in Australia and the U.S.A.
Gov't Doc #: 15881594
Journal: Preparative biochemistry & biotechnology
Type: Journal Article
Subjects: Antigens, Neoplasm.biosynthesis.genetics.isolation & purification.therapeutic use
Cancer Vaccines.biosynthesis.genetics.isolation & purification
Drug Industry.standards
Guidelines as Topic
Membrane Proteins.biosynthesis.genetics.isolation & purification.therapeutic use
Protein Engineering.methods.standards
Quality Assurance, Health Care.standards
Recombinant Proteins.biosynthesis.isolation & purification
Reference Standards
Appears in Collections:Journal articles

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