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https://ahro.austin.org.au/austinjspui/handle/1/9923| Title: | Recombinant NY-ESO-1 cancer antigen: production and purification under cGMP conditions. | Austin Authors: | Murphy, R;Green, S;Ritter, G;Cohen, L;Ryan, D;Woods, W;Rubira, M;Cebon, Jonathan S ;Davis, Ian D;Sjolander, A;Kypridis, A;Kalnins, Renate M;McNamara, M;Moloney, M B;Ackland, J;Cartwright, Glenn A;Rood, J;Dumsday, G;Healey, K;Maher, D;Maraskovsky, Eugene;Chen, Y T;Hoffman, E W;Old, Lloyd J;Scott, Andrew M | Affiliation: | roger.murphy@ludwig.edu.au Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Austin Hospital, Heidelberg, Australia |
Issue Date: | 2005 | Publication information: | Preparative Biochemistry & Biotechnology; 35(2): 119-34 | Abstract: | The cancer-testis antigen, NY-ESO-1, has been engineered into a bacterial expression plasmid which incorporates a His6-tag. The plasmid was transfected into E. coli strain BL21 and Master and Working cell banks generated from this expression system. Three 15-litre fermentations were performed under cGMP (code of Good Manufacturing Practice) conditions and the crude NY-ESO-1 tagged protein isolated as solubilised inclusion bodies. A three-step cGMP chromatography process (immobilised metal affinity, anion exchange, and hydrophobic interaction) was utilised to purify the protein. The purified NY-ESO-1 is being used in early stage human cancer vaccine trials in Australia and the U.S.A. | Gov't Doc #: | 15881594 | URI: | https://ahro.austin.org.au/austinjspui/handle/1/9923 | Journal: | Preparative biochemistry & biotechnology | URL: | https://pubmed.ncbi.nlm.nih.gov/15881594 | Type: | Journal Article | Subjects: | Antigens, Neoplasm.biosynthesis.genetics.isolation & purification.therapeutic use Australia Cancer Vaccines.biosynthesis.genetics.isolation & purification Drug Industry.standards Guidelines as Topic Humans Membrane Proteins.biosynthesis.genetics.isolation & purification.therapeutic use Protein Engineering.methods.standards Quality Assurance, Health Care.standards Recombinant Proteins.biosynthesis.isolation & purification Reference Standards |
| Appears in Collections: | Journal articles |
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