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Title: Regulation of expression of the receptors controlling gastric acidity.
Austin Authors: Kolivas, Sotirios;Shulkes, Arthur
Affiliation: Department of Surgery, Austin and Repatriation Medical Centre, University of Melbourne, Heidelberg, Victoria 3084, Australia
Issue Date: 15-Sep-2004
Publication information: Regulatory Peptides; 121(1-3): 1-9
Abstract: Gastric acid secretion is regulated by the stimulatory effects of gastrin, histamine and acetylcholine and the inhibitory actions of somatostatin on their respective receptors. We proposed that the expression of these receptors could be regulated at the transcription level by agonists and antagonists known to effect acid secretion. A quantitative "real-time" PCR method was used to determine changes in mRNA expression for these receptors. The agonists, pentagastrin and histamine, and the H2 antagonist, ranitidine, were infused over a 6 h period to conscious sheep. Blood, antral and fundic tissue samples were taken for analysis. Both pentagastrin and histamine resulted in elevated plasma somatostatin concentrations during the treatment. Ranitidine stimulated a fourfold increase in plasma gastrin while histamine caused a transient decrease. Except for an increase in antral gastrin following ranitidine infusion, there was no significant change in gastric gastrin and somatostatin concentration. Histamine (H2) receptor mRNA expression in the antrum was significantly increased by pentagastrin and decreased by ranitidine. Pentagastrin also stimulated a significant increase in the level of muscarinic (M3) receptor mRNA in the antrum. Antral somatostatin II receptor mRNA was significantly decreased by histamine. In the fundus, pentagastrin infusion resulted in a significant increase in histamine receptor mRNA and a decrease in the muscarinic receptor mRNA. This work demonstrates that the receptors involved in the regulation of acid secretion can be regulated by local events.
Gov't Doc #: 15256267
DOI: 10.1016/j.regpep.2003.09.009
Journal: Regulatory peptides
Type: Journal Article
Subjects: Animals
Gastric Acid.metabolism
Gene Expression Regulation
Receptor, Cholecystokinin B.genetics
Receptor, Muscarinic M3.genetics
Receptors, Histamine H2.genetics
Receptors, Neurotransmitter.genetics
Receptors, Somatostatin.genetics
Appears in Collections:Journal articles

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