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Title: Immunodominant CD4+ responses identified in a patient vaccinated with full-length NY-ESO-1 formulated with ISCOMATRIX adjuvant.
Austin Authors: Chen, Qiyuan;Jackson, Heather M;Parente, Phillip;Luke, Tina;Rizkalla, Mark;Tai, Tsin Yee;Zhu, He-Cheng;Mifsud, Nicole A;Dimopoulos, Nektaria;Masterman, Kelly-Anne;Hopkins, Wendie;Goldie, Heather;Maraskovsky, Eugene;Green, Simon;Miloradovic, Lena;McCluskey, James;Old, Lloyd J;Davis, Ian D;Cebon, Jonathan S ;Chen, Weisan
Affiliation: Ludwig Institute for Cancer Research, Austin Health, 145-163 Studley Road, Heidelberg, Victoria 3084, Australia
Issue Date: 14-Jun-2004
Publication information: Proceedings of the National Academy of Sciences of the United States of America 2004; 101(25): 9363-8
Abstract: There is increasing evidence showing the involvement of CD4(+) T cells in initiating and maintaining antitumor immune responses. NY-ESO-1 is expressed by various tumors but not normal tissues except testis. We conducted a cancer clinical trial by using full-length NY-ESO-1 protein formulated with ISCOMATRIX adjuvant and injected into patients intramuscularly. Autologous dendritic cells pulsed with NY-ESO-1 ISCOMATRIX in combination with overlapping synthetic peptides were used to identify immunodominant T cells from a vaccinated patient. We show here the identification and characterization of two novel CD4(+) T cell epitopes. T cells specific to these epitopes not only recognized autologous dendritic cells loaded with NY-ESO-1 but also NY-ESO-1-expressing tumor cell lines treated with IFN-gamma. One of the two responses identified was greater than the previously identified immunodominant HLA-DP4-restricted response and correlated with NY-ESO-1-specific CD8(+) T cell induction after vaccination. This T cell response was vaccinated in most patients who expressed HLA-DR2. This study has systematically surveyed patients vaccinated with full-length tumor antigen for a vaccinated CD4 helper T cell response.
Gov't Doc #: 15197261
DOI: 10.1073/pnas.0403271101
Type: Journal Article
Subjects: Amino Acid Sequence
Antigens, Neoplasm.immunology
CD4-Positive T-Lymphocytes.immunology
CD8-Positive T-Lymphocytes.immunology
Cell Line, Tumor
Cells, Cultured
Lymphocyte Activation
Major Histocompatibility Complex.immunology
Membrane Proteins.immunology
Molecular Sequence Data
Vaccines, Synthetic.immunology
Appears in Collections:Journal articles

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