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|Title:||Immunodominant CD4+ responses identified in a patient vaccinated with full-length NY-ESO-1 formulated with ISCOMATRIX adjuvant.||Austin Authors:||Chen, Qiyuan;Jackson, Heather M;Parente, Phillip;Luke, Tina;Rizkalla, Mark;Tai, Tsin Yee;Zhu, He-Cheng;Mifsud, Nicole A;Dimopoulos, Nektaria;Masterman, Kelly-Anne;Hopkins, Wendie;Goldie, Heather;Maraskovsky, Eugene;Green, Simon;Miloradovic, Lena;McCluskey, James;Old, Lloyd J;Davis, Ian D;Cebon, Jonathan S ;Chen, Weisan||Affiliation:||Ludwig Institute for Cancer Research, Austin Health, 145-163 Studley Road, Heidelberg, Victoria 3084, Australia||Issue Date:||14-Jun-2004||Publication information:||Proceedings of the National Academy of Sciences of the United States of America 2004; 101(25): 9363-8||Abstract:||There is increasing evidence showing the involvement of CD4(+) T cells in initiating and maintaining antitumor immune responses. NY-ESO-1 is expressed by various tumors but not normal tissues except testis. We conducted a cancer clinical trial by using full-length NY-ESO-1 protein formulated with ISCOMATRIX adjuvant and injected into patients intramuscularly. Autologous dendritic cells pulsed with NY-ESO-1 ISCOMATRIX in combination with overlapping synthetic peptides were used to identify immunodominant T cells from a vaccinated patient. We show here the identification and characterization of two novel CD4(+) T cell epitopes. T cells specific to these epitopes not only recognized autologous dendritic cells loaded with NY-ESO-1 but also NY-ESO-1-expressing tumor cell lines treated with IFN-gamma. One of the two responses identified was greater than the previously identified immunodominant HLA-DP4-restricted response and correlated with NY-ESO-1-specific CD8(+) T cell induction after vaccination. This T cell response was vaccinated in most patients who expressed HLA-DR2. This study has systematically surveyed patients vaccinated with full-length tumor antigen for a vaccinated CD4 helper T cell response.||Gov't Doc #:||15197261||URI:||http://ahro.austin.org.au/austinjspui/handle/1/9752||DOI:||10.1073/pnas.0403271101||URL:||https://pubmed.ncbi.nlm.nih.gov/15197261||Type:||Journal Article||Subjects:||Amino Acid Sequence
Cell Line, Tumor
Major Histocompatibility Complex.immunology
Molecular Sequence Data
|Appears in Collections:||Journal articles|
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