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Title: Isolation and characterization of cDNA clones for mouse Ly-9.
Austin Authors: Sandrin, Mauro S ;Gumley, T P;Henning, M M;Vaughan, Hilary A;Gonez, L J;Trapani, Joseph A;McKenzie, Ian F C
Affiliation: Molecular Immunogenetics Laboratory, Austin Research Institute, Austin Hospital Heidelberg, Victoria, Australia
Issue Date: 1-Sep-1992
Publication information: Journal of Immunology (baltimore, Md. : 1950); 149(5): 1636-41
Abstract: We describe the production and characterization of a mouse mAb, S-450-33.2, recognizing the Ly-9.2 specificity. This mAb was used to purify Ly-9 molecules from lymphoid cell lines, and the amino-terminal amino acids were determined. The mAb was also used in a eukaryotic expression system, to isolate cDNA clones encoding Ly-9. Analysis of RNA showed that Ly-9 expression is lymphocyte specific, as determined by the presence of a single hybridizing 2.4-kb species found only in lymphoid cells. Genomic DNA analysis indicated that Ly-9 is encoded by a single-copy gene of 10 to 15 kb. The predicted polypeptide belongs to the Ig superfamily of cell surface molecules with four extracellular Ig-like domains, i.e., a non-disulfide-bonded V domain, a truncated C2 domain with two disulfide bonds, a second non-disulfide-bonded V domain, and a truncated C2 domain with two disulfide bonds (V-C2-V-C2). The sequence data also support the view that Ly-9 belongs to the subgroup of the Ig superfamily that includes Bcm-1, CD2, and LFA-3.
Gov't Doc #: 1506686
Type: Journal Article
Subjects: Amino Acid Sequence
Antibodies, Monoclonal.biosynthesis
Antigens, Ly.analysis.chemistry.genetics
Base Sequence
Blotting, Northern
Blotting, Southern
DNA.chemistry.isolation & purification
Genes, Immunoglobulin
Molecular Sequence Data
Appears in Collections:Journal articles

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