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Title: Effect of interstitial laser hyperthermia in a murine model of colorectal liver metastases: scanning electron microscopic study.
Austin Authors: Muralidharan, Vijayaragavan ;Nikfarjam, Mehrdad ;Malcontenti-Wilson, Caterina;Christophi, Christopher 
Affiliation: Department of Surgery, University of Melbourne, Austin Hospital, Studley Road, Heidelberg, 3084 Melbourne, Victoria, Australia
Issue Date: 5-Dec-2003
Publication information: World Journal of Surgery 2003; 28(1): 33-7
Abstract: Interstitial laser hyperthermia (ILH) is an in situ ablative technique used to treat colorectal liver metastases. The relatively high recurrence of tumor after treatment by ILH may be related to incomplete destruction. Little is known about the effectiveness of ILH for destroying tumor microvasculature. The aim of this study was to define the changes to the microvascular architecture of tumors after treatment with ILH, specifically focusing on the completeness of tumor vasculature destruction. An intrasplenic induction model of liver metastases in 4- to 6-week-old male inbred CBA mice was used. Laser hyperthermia was applied to liver and tumor tissue using a bare optical quartz fiber from a Medilas Fibertom 4100 Nd:YAG surgical laser generator. The animals underwent microvascular corrosion casting of the livers immediately after application of ILH. Microvascular casts were then prepared and studied by scanning electron microscopy. ILH produced complete, uniform destruction of the tumor microvasculature with compete hemostasis. Blood flow in vessels larger than 100 microm diameter had a relatively protective effect, although ILH was able to overcome this barrier effectively by increasing the energy applied. ILH produces complete destruction of tumor microvasculature with hemostasis. The protective effect of blood flow in larger vessels can be overcome by the appropriate use of higher energy levels.
Gov't Doc #: 14648042
DOI: 10.1007/s00268-003-6973-0
Type: Journal Article
Subjects: Animals
Colorectal Neoplasms.blood supply.pathology.ultrastructure
Disease Models, Animal
Hyperthermia, Induced.methods
Laser Therapy
Liver Neoplasms.secondary.therapy.ultrastructure
Mice, Inbred CBA
Microscopy, Electron, Scanning
Appears in Collections:Journal articles

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