Please use this identifier to cite or link to this item:
Title: Regulation of angiotensin II receptors in the prostate of the transgenic (mRen-2)27 rat: effect of angiotensin-converting enzyme inhibition.
Austin Authors: Fabiani, Maurice E;Hawkes, David J;Frauman, Albert G ;Tikellis, Christos;Johnston, Colin I;Wilkinson-Berka, Jennifer L
Affiliation: Department of Medicine, University of Melbourne, Austin and Repatriation Medical Centre, Heidelberg, VIC 3084, Australia
Issue Date: 1-Jun-2003
Publication information: The International Journal of Biochemistry & Cell Biology; 35(6): 973-83
Abstract: We examined the regulation/expression of angiotensin II (Ang II) receptors in the transgenic (TG) (mRen-2)27 rat compared to the normal Sprague-Dawley (SD) rat. Ang II receptor binding and mRNA expression were determined by quantitative autoradiography and real-time PCR, respectively. Ang II receptors in the rat prostate rat were of the AT(1) receptor subtype and were significantly reduced in the prostate of the TG rat compared to the normal SD rat. However, AT(1) receptor binding was significantly higher in the prostate of the TG rat treated with the ACE inhibitor lisinopril compared to the untreated TG rat and comparable to the control SD rat. In contrast to the protein, AT(1) receptor mRNA expression was not reduced in the prostate of the TG rat compared to the SD rat. However, AT(1) receptor mRNA was markedly reduced in the prostate of the lisinopril-treated TG rat compared to the untreated TG rat or control SD rat. In conclusion, the findings suggest that AT(1) receptors are present in the rat prostate at a protein level and are subject to down-regulation in the TG rat which may be due to receptor internalisation as a consequence of receptor hyper-stimulation by increased local tissue levels of Ang II. Moreover, AT(1) receptor protein and mRNA expression in the prostate may be inversely modulated.
Gov't Doc #: 12676181
Type: Journal Article
Subjects: Adaptor Proteins, Signal Transducing
Angiotensin-Converting Enzyme Inhibitors.pharmacology
Animals, Genetically Modified
Carrier Proteins.biosynthesis
Peptidyl-Dipeptidase A.metabolism
Prostate.drug effects.metabolism
RNA, Messenger.biosynthesis
Rats, Sprague-Dawley
Receptor, Angiotensin, Type 1
Receptors, Angiotensin.biosynthesis.metabolism
Reverse Transcriptase Polymerase Chain Reaction
Appears in Collections:Journal articles

Show full item record

Page view(s)

checked on Dec 7, 2022

Google ScholarTM


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.