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Title: | Periwound dopaminergic sprouting is dependent on numbers of wound macrophages. | Austin Authors: | Batchelor, Peter Egerton;Porritt, Michelle J;Nilsson, S K;Bertoncello, I;Donnan, Geoffrey A ;Howells, David William | Affiliation: | Departments of Medicine and Neurology, University of Melbourne, Austin and Repatriation Medical Centre, Heidelberg Victoria 3084, Australia | Issue Date: | 1-Mar-2002 | Publication information: | The European Journal of Neuroscience; 15(5): 826-32 | Abstract: | Injury to many regions of the central nervous system, including the striatum, results in a periwound or 'abortive' sprouting response. In order to directly evaluate whether macrophages play an important role in stimulating periwound sprouting, osteopetrotic (op/op) mice, which when young are deficient in a variety of macrophage subtypes, were given striatal wounds and the degree of dopaminergic sprouting subsequently assessed. Two weeks postinjury, significantly fewer wound macrophages were present in the striata of op/op mice compared with controls (144 +/- 30.1 in op/op mice vs. 416.6 +/- 82.3 in controls, P < 0.005, analysis performed on a section transecting the middle of the wound). Dopamine transporter immunohistochemistry revealed a marked decrease in the intensity of periwound sprouting in the op/op group of animals. Quantification of this effect using [H3]-mazindol autoradiography confirmed that periwound sprouting was reduced significantly in the op/op mice compared with controls (71.4 +/- 21.7 fmol/mg protein in op/op mice vs. 210.7 +/- 27.1 fmol/mg protein in controls, P < 0.0005). In the two groups of animals the magnitude of the sprouting response in individuals was closely correlated with the number of wound macrophages (R = 0.83, R2 = 0.69). Our findings provide strong support for the crucial involvement of macrophages in inducing dopaminergic sprouting after striatal injury. | Gov't Doc #: | 11906524 | URI: | https://ahro.austin.org.au/austinjspui/handle/1/9392 | Journal: | The European journal of neuroscience | URL: | https://pubmed.ncbi.nlm.nih.gov/11906524 | Type: | Journal Article | Subjects: | Adrenergic Uptake Inhibitors.diagnostic use Animals Brain Injuries.metabolism.physiopathology Carboxylesterase Carboxylic Ester Hydrolases.metabolism Cell Count Corpus Striatum.cytology.injuries.metabolism Denervation Dopamine.metabolism Growth Cones.metabolism.ultrastructure Macrophage-1 Antigen.metabolism Macrophages.cytology.metabolism Mazindol.diagnostic use Mice Mice, Inbred C57BL Mice, Mutant Strains Nerve Regeneration.physiology Osteopetrosis.genetics.immunology Presynaptic Terminals.metabolism.ultrastructure Tritium.diagnostic use Wound Healing.physiology |
Appears in Collections: | Journal articles |
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