Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/34435
Title: High-performance enrichment-based genome sequencing to support the investigation of hepatitis A virus outbreaks.
Austin Authors: Zufan, Sara E;Mercoulia, Karolina;Kwong, Jason C ;Judd, Louise M;Howden, Benjamin P ;Seemann, Torsten;Stinear, Timothy P
Affiliation: The Center for Pathogen Genomics, The University of Melbourne, Melbourne, Victoria, Australia.;Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.;Microbiological Diagnostic Unit Public Health Laboratory, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Infectious Diseases
Doherty Applied Microbial Genomics, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Microbiological Diagnostic Unit Public Health Laboratory, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Issue Date: 11-Jan-2024
Date: 2023
Publication information: Microbiology Spectrum 2024-01-11; 12(1)
Abstract: This proof-of-concept study introduces a hybrid capture oligo panel for whole-genome sequencing of all six human pathogenic hepatitis A virus (HAV) subgenotypes, exhibiting a higher sensitivity than some conventional genotyping assays. The ability of hybrid capture to enrich multiple targets allows for a single, streamlined workflow, thus facilitating the potential harmonization of molecular surveillance of HAV with other enteric viruses. Even challenging sample matrices can be accommodated, making them suitable for broad implementation in clinical and public health laboratories. This innovative approach has significant implications for enhancing multijurisdictional outbreak investigations as well as our understanding of the global diversity and transmission dynamics of HAV.
URI: https://ahro.austin.org.au/austinjspui/handle/1/34435
DOI: 10.1128/spectrum.02834-23
ORCID: 0000-0002-3606-0297
0000-0003-0150-123X
Journal: Microbiology Spectrum
Start page: e0283423
PubMed URL: 38018979
ISSN: 2165-0497
Type: Journal Article
Subjects: culture-independent diagnostics
food-borne surveillance
molecular epidemiology
public health genomics
whole-genome sequencing
Appears in Collections:Journal articles

Show full item record

Page view(s)

20
checked on Oct 8, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.