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https://ahro.austin.org.au/austinjspui/handle/1/34435| Title: | High-performance enrichment-based genome sequencing to support the investigation of hepatitis A virus outbreaks. | Austin Authors: | Zufan, Sara E;Mercoulia, Karolina;Kwong, Jason C ;Judd, Louise M;Howden, Benjamin P ;Seemann, Torsten;Stinear, Timothy P | Affiliation: | The Center for Pathogen Genomics, The University of Melbourne, Melbourne, Victoria, Australia.;Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia. Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.;Microbiological Diagnostic Unit Public Health Laboratory, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia. Infectious Diseases Doherty Applied Microbial Genomics, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia. Microbiological Diagnostic Unit Public Health Laboratory, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia. |
Issue Date: | 11-Jan-2024 | Date: | 2023 | Publication information: | Microbiology Spectrum 2024-01-11; 12(1) | Abstract: | This proof-of-concept study introduces a hybrid capture oligo panel for whole-genome sequencing of all six human pathogenic hepatitis A virus (HAV) subgenotypes, exhibiting a higher sensitivity than some conventional genotyping assays. The ability of hybrid capture to enrich multiple targets allows for a single, streamlined workflow, thus facilitating the potential harmonization of molecular surveillance of HAV with other enteric viruses. Even challenging sample matrices can be accommodated, making them suitable for broad implementation in clinical and public health laboratories. This innovative approach has significant implications for enhancing multijurisdictional outbreak investigations as well as our understanding of the global diversity and transmission dynamics of HAV. | URI: | https://ahro.austin.org.au/austinjspui/handle/1/34435 | DOI: | 10.1128/spectrum.02834-23 | ORCID: | 0000-0002-3606-0297 0000-0003-0150-123X |
Journal: | Microbiology Spectrum | Start page: | e0283423 | PubMed URL: | 38018979 | ISSN: | 2165-0497 | Type: | Journal Article | Subjects: | culture-independent diagnostics food-borne surveillance molecular epidemiology public health genomics whole-genome sequencing |
| Appears in Collections: | Journal articles |
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