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Title: Circulating microbial cell-free DNA is increased during neutropenia following hematopoietic stem cell transplantation.
Austin Authors: Blair, Lily M;Akhund-Zade, Jamilla;Katsamakis, Zoe A;Smibert, Olivia C ;Wolfe, Alex E;Giardina, Paul;Slingerland, John;Bercovici, Sivan;Perales, Miguel-Angel;Taur, Ying;van den Brink, Marcel R M;Peled, Jonathan U;Markey, Kate A
Affiliation: Karius, Inc, Redwood City, California, United States.
Karius, Inc, Redwood City, California, United States.
Memorial Sloan Kettering Cancer Center, New York, New York, United States.
Austin Health
Fred Hutchinson Cancer Center, Seattle, Washington, United States.
Flatiron Health, New York, New York, United States.
Memorial Sloan Kettering Cancer Center, New York, New York, United States.
Memorial Sloan Kettering Cancer Center, New York, New York, United States.
University of Washington, United States.
Issue Date: 14-Nov-2023
Date: 2023
Publication information: Blood Advances 2023-11-14; 7(21)
Abstract: We used a next-generation sequencing platform to characterize microbial cell-free DNA (mcfDNA) in plasma samples from patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HCT). In this observational study, we sought to characterize plasma mcfDNA in order to understand its potential association with the immunologic complications of transplantation. We compared serially-collected patient samples with plasma collected from healthy control subjects. We observed changes in total mcfDNA burden in plasma after transplantation, which was most striking during the early post-transplant neutropenic phase. This elevation could be attributed to a number of specific bacterial taxa, including Veillonella, Bacteroides and Prevotella (genus level). For an additional cohort of patients, we compared mcfDNA from plasma with 16S-rRNA sequencing data from stool samples collected at matched time points. In a number of patients, we confirmed that mcfDNA derived from specific microbial taxa (e.g. Enterococcus) could also be observed in the matched stool sample. Quantification of mcfDNA may generate novel insights into mechanisms by which the intestinal microbiome influences systemic cell populations and thus has been associated with outcomes for cancer patients.
DOI: 10.1182/bloodadvances.2023010208
ORCID: 0000-0001-5589-8258
Journal: Blood Advances
PubMed URL: 37399491
ISSN: 2473-9537
Type: Journal Article
Appears in Collections:Journal articles

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