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Title: Longitudinal quantification of mouse gastric tumor organoid viability and growth using luminescence and microscopy.
Austin Authors: Morrow, Riley J;Ernst, Matthias ;Poh, Ashleigh R
Affiliation: Olivia Newton-John Cancer Research Institute
School of Cancer Medicine, La Trobe University, Heidelberg, VIC 3084, Australia.
Issue Date: 10-Feb-2023 2023
Publication information: STAR Protocols 2023; 4(1)
Abstract: Tumor-derived organoids are valuable for testing anti-cancer drugs in┬ávitro, but existing lysis-based protocols for viability measurement are laborious and restricted at a single time point. Here, we provide a lysis-free protocol for longitudinal and rapid assessment of mouse gastric tumor organoid viability and growth. We describe organoid plating, viability assessment via luminescence measurement, quantification of organoid growth by microscopy imaging, and treatment of organoids with test compounds to evaluate the effects on viability and growth at various time points.
DOI: 10.1016/j.xpro.2023.102110
Journal: STAR Protocols
Start page: 102110
PubMed URL: 36853665
ISSN: 2666-1667
Type: Journal Article
Subjects: Cancer
Cell Biology
Cell culture
Cell-based Assays
Model Organisms
Appears in Collections:Journal articles

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