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Title: An intrasplenic injection model of pancreatic cancer metastasis to the liver in mice.
Austin Authors: O'Brien, Megan;Ernst, Matthias ;Poh, Ashleigh R
Affiliation: Olivia Newton-John Cancer Research Institute and La Trobe University School of Cancer Medicine, Heidelberg, VIC 3084, Australia.
Olivia Newton-John Cancer Research Institute
Issue Date: 17-Mar-2023
Date: 2023
Publication information: STAR Protocols 2023-03-17; 4(1)
Abstract: Here, we provide a protocol for an intrasplenic injection model to establish pancreatic tumors in the mouse liver. We describe the steps to inject tumor cells into mouse spleen and to perform a splenectomy, followed by animal recovery and end point analysis of tumors in the liver. This model allows rapid and reproducible tumor growth in a clinically relevant metastatic site, providing a platform to evaluate the efficacy of anti-cancer drugs. This technique can be expanded to other cancer cell lines. For complete details on the use and execution of this protocol, please refer to Poh et al. (2022).1.
DOI: 10.1016/j.xpro.2022.102021
Journal: STAR Protocols
Start page: 102021
PubMed URL: 36638017
PubMed URL:
ISSN: 2666-1667
Type: Journal Article
Subjects: Cancer
Cell Biology
Cell culture
Model Organisms
Appears in Collections:Journal articles

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