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|Title:||An intrasplenic injection model of pancreatic cancer metastasis to the liver in mice.||Austin Authors:||O'Brien, Megan;Ernst, Matthias ;Poh, Ashleigh R||Affiliation:||Olivia Newton-John Cancer Research Institute and La Trobe University School of Cancer Medicine, Heidelberg, VIC 3084, Australia.
Olivia Newton-John Cancer Research Institute
|Issue Date:||17-Mar-2023||Date:||2023||Publication information:||STAR Protocols 2023-03-17; 4(1)||Abstract:||Here, we provide a protocol for an intrasplenic injection model to establish pancreatic tumors in the mouse liver. We describe the steps to inject tumor cells into mouse spleen and to perform a splenectomy, followed by animal recovery and end point analysis of tumors in the liver. This model allows rapid and reproducible tumor growth in a clinically relevant metastatic site, providing a platform to evaluate the efficacy of anti-cancer drugs. This technique can be expanded to other cancer cell lines. For complete details on the use and execution of this protocol, please refer to Poh et al. (2022).1.||URI:||https://ahro.austin.org.au/austinjspui/handle/1/31962||DOI:||10.1016/j.xpro.2022.102021||ORCID:||Journal:||STAR Protocols||Start page:||102021||PubMed URL:||36638017||PubMed URL:||https://pubmed.ncbi.nlm.nih.gov/36638017/||ISSN:||2666-1667||Type:||Journal Article||Subjects:||Cancer
|Appears in Collections:||Journal articles|
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checked on Dec 3, 2023
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