| Title |
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Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry. |
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| Publication Date |
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| Author(s) |
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| Subject |
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Multiplex immunohistochemistry |
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Tyramide signal amplification (TSA) |
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| Type of document |
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| OrcId |
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#PLACEHOLDER_PARENT_METADATA_VALUE# |
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#PLACEHOLDER_PARENT_METADATA_VALUE# |
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#PLACEHOLDER_PARENT_METADATA_VALUE# |
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| DOI |
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10.1007/978-1-0716-2811-9_20 |
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| Abstract |
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Multiplex immunohistochemistry (mIHC) facilitates the simultaneous detection of various immune cell markers on a single tissue section. Here, we describe a protocol for an mIHC staining workflow using specific antibodies against CD4, CD8α, FOXP3, and B220 to identify distinct lymphocyte populations including T and B cells. This staining strategy can be adapted to include other cell markers to evaluate the immune contexture in murine tissues. |
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| Link |
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| Citation |
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Methods in Molecular Biology 2023 |
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| Jornal Title |
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Methods in Molecular Biology |
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| ISSN |
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