Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/29719
Title: Neuronal androgen receptor is required for activity dependent enhancement of peripheral nerve regeneration.
Austin Authors: Ward, Patricia J;Davey, Rachel A;Zajac, Jeffrey D ;English, Arthur W
Affiliation: Medicine (University of Melbourne)
Department of Cell Biology, Emory University School of Medicine, Atlanta, GA, USA..
Issue Date: May-2021
Date: 2021
Publication information: Developmental Neurobiology 2021; 81(4): 411-423
Abstract: Neuronal activity after nerve injury can enhance axon regeneration and the restoration of function. The mechanism for this enhancement relies in part on hormone receptors, and we previously demonstrated that systemic androgen receptor antagonism blocked the effect of exercise or electrical stimulation on enhancing axon regeneration after nerve injury in both sexes. Here, we tested the hypothesis that the site of this androgen receptor signaling is both neuronal and involves the classical, genomic signaling pathway. In vivo, dorsal root ganglion neurons successfully regenerate in response to activity-dependent neuronal activation, and conditional deletion of the DNA-binding domain of the androgen receptor in adults blocks this effect in males and females. Motoneurons in males and females also respond in this manner, but we also observed a sex difference. In vitro, cultured sensory dorsal root ganglion neurons respond to androgens via traditional androgen receptor signaling mechanisms leading to enhanced neurite growth and did not respond to a testosterone conjugate that is unable to cross the cell membrane. Given our previous observation of a requirement for activity-dependent androgen receptor signaling to promote regeneration in both sexes, we interpret our results to indicate that genomic neuronal androgen receptor signaling is required for activity-dependent axon regeneration in both sexes.
URI: https://ahro.austin.org.au/austinjspui/handle/1/29719
DOI: 10.1002/dneu.22826
ORCID: 0000-0002-5379-3644
0000-0001-5121-0209
0000-0003-3933-5708
Journal: Developmental neurobiology
PubMed URL: 33864349
PubMed URL: https://pubmed.ncbi.nlm.nih.gov/33864349/
Type: Journal Article
Subjects: androgen receptor
axon regeneration
dorsal root ganglia
electrical stimulation
motoneuron
nerve injury
testosterone
Appears in Collections:Journal articles

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