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Title: | Lung-resident memory B cells established after pulmonary influenza infection display distinct transcriptional and phenotypic profiles. | Austin Authors: | Tan, Hyon-Xhi;Juno, Jennifer A;Esterbauer, Robyn;Kelly, Hannah G;Wragg, Kathleen M;Konstandopoulos, Penny;Alcantara, Sheilajen;Alvarado, Carolina;Jones, Robert M ;Starkey, Graham M ;Wang, Boa Zhong;Yoshino, Osamu ;Tiang, Thomas;Grayson, M Lindsay ;Opdam, Helen I ;D'Costa, Rohit;Vago, Angela ;Mackay, Laura K;Gordon, Claire L ;Masopust, David;Groom, Joanna R;Kent, Stephen J;Wheatley, Adam K | Affiliation: | Intensive Care Infectious Diseases Surgery Division of Immunology, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia Central Clinical School, Monash University, Melbourne, Victoria 3004, Australia ARC Centre for Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Parkville, Victoria 3010, Australia DonateLife Victoria, Carlton, Victoria 3053, Australia Intensive Care Unit, The Royal Melbourne Hospital, Parkville, Victoria 3050, Australia Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia DonateLife, The Australian Organ and Tissue Authority, Canberra, Australian Capital Territory 2601, Australia Department of Microbiology and Immunology, University of Minnesota, Minneapolis, MN, USA Center for Immunology, University of Minnesota, Minneapolis, MN, USA Melbourne Sexual Health Centre and Department of Infectious Diseases, Alfred Hospital, Melbourne, Australia |
Issue Date: | 28-Jan-2022 | Date: | 2022-01-28 | Publication information: | Science Immunology 2022; 7(67): eabf5314 | Abstract: | Recent studies have established that memory B cells, largely thought to be circulatory in the blood, can take up long-term residency in inflamed tissues, analogous to widely described tissue-resident T cells. The dynamics of recruitment and retention of memory B cells to tissues and their immunological purpose remains unclear. Here, we characterized tissue-resident memory B cells (BRM) that are stably maintained in the lungs of mice after pulmonary influenza infection. Influenza-specific BRM were localized within inducible bronchus-associated lymphoid tissues (iBALTs) and displayed transcriptional signatures distinct from classical memory B cells in the blood or spleen while showing partial overlap with memory B cells in lung-draining lymph nodes. We identified lung-resident markers, including elevated expression of CXCR3, CCR6, and CD69, on hemagglutinin (HA)- and nucleoprotein (NP)-specific lung BRM. We found that CCR6 facilitates increased recruitment and/or retention of BRM in lungs and differentiation into antibody-secreting cells upon recall. Although expression of CXCR3 and CCR6 was comparable in total and influenza-specific memory B cells isolated across tissues of human donors, CD69 expression was higher in memory B cells from lung and draining lymph nodes of human organ donors relative to splenic and PBMC-derived populations, indicating that mechanisms underpinning BRM localization may be evolutionarily conserved. Last, we demonstrate that human memory B cells in lungs are transcriptionally distinct to populations in lung-draining lymph nodes or PBMCs. These data suggest that BRM may constitute a discrete component of B cell immunity, positioned at the lung mucosa for rapid humoral response against respiratory viral infections. | URI: | https://ahro.austin.org.au/austinjspui/handle/1/28684 | DOI: | 10.1126/sciimmunol.abf5314 | ORCID: | 0000-0003-1064-3934 0000-0002-9072-1017 0000-0002-7091-0048 0000-0002-4652-5609 0000-0002-7798-9182 0000-0002-8415-5565 0000-0002-4285-1343 0000-0003-1219-5362 0000-0001-7702-3479 0000-0003-2313-2623 0000-0002-8496-6632 0000-0001-5172-4728 0000-0002-9440-3884 0000-0001-5251-7835 0000-0002-8539-4891 0000-0002-5593-9387 0000-0002-3261-3149 |
Journal: | Science Immunology | PubMed URL: | 35089815 | PubMed URL: | https://pubmed.ncbi.nlm.nih.gov/35089815/ | Type: | Journal Article |
Appears in Collections: | Journal articles |
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